Cloned (Comment) | Organism |
---|---|
gene Cs(S)-THBO, DNA and amino acid sequence determination and analysis, real-time quantitative PCR analysis, tissue expression profiling analyses, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Corydalis saxicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
43000 | - |
x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydroberberine + 2 O2 | Corydalis saxicola | - |
berberine + 2 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corydalis saxicola | G0XRK4 | gene Cs(S)-THBO | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Corydalis saxicola | - |
leaf | - |
Corydalis saxicola | - |
additional information | the enzyme is constitutively expressed in roots, stems, leaves and flowers, with the highest expression level in roots, real-time quantitative PCR analysis, tissue expression profiling analyses, overview | Corydalis saxicola | - |
root | - |
Corydalis saxicola | - |
stem | - |
Corydalis saxicola | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydroberberine + 2 O2 | - |
Corydalis saxicola | berberine + 2 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
Synonyms | Comment | Organism |
---|---|---|
(S)-tetrahydroberberine oxidase | - |
Corydalis saxicola |
(S)-THB oxidase | - |
Corydalis saxicola |
General Information | Comment | Organism |
---|---|---|
metabolism | (S)-tetrahydroberberine oxidase is the last enzyme of benzylisoquinoline alkaloids pathway which catalyzes the dehydrogenation of four hydrogen atoms of (S)-THB to produce berberine, the final step of berberine biosynthesis | Corydalis saxicola |