Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
F188A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Pre-steady state kinetic analysis | Plasmodium falciparum |
F227A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
H185A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Pre-steady state kinetic analysis | Plasmodium falciparum |
I272A | decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
L531A | decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
R265A | largest effect on Km for CoQ substrate among the mutants tested. Pre-steady state kinetic analysis | Plasmodium falciparum |
Y528A | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
Y528F | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
Y528W | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.8 | - |
coenzyme Q1 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
8.8 | - |
coenzyme QD | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
11 | - |
coenzyme Q1 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
12 | - |
coenzyme QD | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
13 | - |
coenzyme QD | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
15 | - |
coenzyme Q1 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
16 | - |
coenzyme Q1 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
16 | - |
coenzyme QD | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
16 | - |
coenzyme Q1 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
17 | - |
coenzyme Q1 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
20 | - |
coenzyme QD | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
20 | - |
coenzyme QD | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
20 | - |
coenzyme Q1 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
22 | - |
coenzyme Q1 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
26 | - |
coenzyme QD | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
34 | - |
coenzyme QD | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
38 | - |
coenzyme Q1 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
45 | - |
coenzyme QD | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
48 | - |
coenzyme Q1 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
53 | - |
coenzyme QD | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q08210 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + coenzyme Q1 | coenzyme Q1 possesses a prenyl tail | Plasmodium falciparum | orotate + reduced coenzyme Q1 | - |
? | |
dihydroorotate + coenzyme QD | coenzyme QD possesses a decyl tail | Plasmodium falciparum | orotate + reduced coenzyme QD | - |
? | |
dihydroorotate + O2 | - |
Plasmodium falciparum | orotate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
O2 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
0.1 | - |
O2 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
0.12 | - |
coenzyme QD | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
0.18 | - |
O2 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
0.19 | - |
coenzyme Q1 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
0.25 | - |
O2 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
0.42 | - |
O2 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
0.43 | - |
O2 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
0.44 | - |
O2 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
0.53 | - |
O2 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
0.57 | - |
O2 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
0.63 | - |
O2 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.2 | - |
coenzyme QD | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.5 | - |
coenzyme Q1 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.8 | - |
coenzyme QD | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.9 | - |
coenzyme Q1 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
2.6 | - |
coenzyme Q1 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
2.6 | - |
coenzyme Q1 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
3.1 | - |
coenzyme Q1 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
3.5 | - |
coenzyme QD | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
4.3 | - |
coenzyme QD | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
5 | - |
coenzyme QD | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
5.1 | - |
coenzyme Q1 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
5.1 | - |
coenzyme Q1 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
6.8 | - |
coenzyme QD | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
7.3 | - |
coenzyme Q1 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
7.8 | - |
coenzyme Q1 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
7.9 | - |
coenzyme QD | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
8.6 | - |
coenzyme QD | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
12 | - |
coenzyme QD | wild-type, pH 8.0, 25°C | Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.049 | - |
wild-type, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
0.087 | - |
mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
0.1 | - |
mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.9 | - |
mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
2.9 | - |
mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
12 | - |
mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide |