Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity | Clostridium sp. | |
phosphate | crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity | Clostridium sp. | |
pyruvate | crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity | Clostridium sp. |
General Stability | Organism |
---|---|
cobinamide cyanide, 0.01 mM, accelerates loss of activity in air | Clostridium sp. |
ethylene glycol high concentrations stabilize | Clostridium sp. |
glycerol, concentrations above 30% stabilize | Clostridium sp. |
Na2S * 9 H2O stabilizes, maximal effect at 0.03% | Clostridium sp. |
stable to freezing and thawing | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
reduced ferredoxin | - |
Clostridium sp. | |
0.3 | - |
reduced methyl viologen | - |
Clostridium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
air, 5°C, 24 h, 50% loss of activity | Clostridium sp. |
Purification (Comment) | Organism |
---|---|
partial | Clostridium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
- |
Clostridium sp. |
Storage Stability | Organism |
---|---|
several months in liquid nitrogen and up to 50 h with argon | Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4,5,6-tetrahydro-6-oxonicotinate + oxidized ferredoxin | oxidized methyl viologen is also substrate | Clostridium sp. | 6-hydroxynicotinate + reduced ferredoxin | - |
r | |
6-hydroxynicotinate + reduced ferredoxin | - |
Clostridium sp. | 6-oxo-1,4,5,6-tetrahydronicotinate + oxidized ferredoxin | - |
r | |
6-hydroxynicotinate + reduced methyl viologen | - |
Clostridium sp. | 6-oxo-1,4,5,6-tetrahydronicotinate + oxidized methyl viologen | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Clostridium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
stable in liquid nitrogen, unstable in air | Clostridium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Clostridium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.2 | pH 5.5: about 60% of activity maximum, pH 8.2: about 20% of activity maximum, rapid decline in activity below pH 5.6 may be partly due to destruction of ferredoxin in acid | Clostridium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Clostridium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | crude enzyme fractions: pyruvate, ferredoxin, CoA and phosphate or arsenate required for maximal activity | Clostridium sp. | |
Ferredoxin | reduced or oxidized, as electron carrier | Clostridium sp. |