Cloned (Comment) | Organism |
---|---|
recombinant expression of chimeric enzyme mutants in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric enzymes composed of portions of the Arabidopsis and the tomato proteins and expression in Escherichia coli, functional complementation of Arabidopsis acd2-2 mutant | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | soluble protein | Arabidopsis thaliana | 9507 | - |
additional information | in young Arabidopsis seedlings, the enzyme is also associated with mitochondria | Arabidopsis thaliana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 35000, precursor enzyme, SDS-PAGE, x * 31000, mature enzyme, SDS-PAGE | Arabidopsis thaliana |
35000 | - |
x * 35000, precursor enzyme, SDS-PAGE, x * 31000, mature enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Arabidopsis thaliana | - |
primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8LDU4 | gene RCCR or ACD2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Arabidopsis thaliana | primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
red chlorophyll catabolite + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | reduction of the C20/C1 double bond of red chlorophyll catabolite is catalyzed by RCCR in a stereospecific manner forming the C1 isomer pFCC-1 | Arabidopsis thaliana | primary fluorescent chlorophyll catabolite + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, precursor enzyme, SDS-PAGE, x * 31000, mature enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtRCCR | - |
Arabidopsis thaliana |
RCC reductase | - |
Arabidopsis thaliana |
RCCR | - |
Arabidopsis thaliana |
red Chl catabolite reductase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | ferredoxin dependent, but appears to lack a metal or flavin cofactor, indicating that electrons are directly transferred from ferredoxin to red chlorophyll catabolite, RCC | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | RCCR is distantly related to a family of bilin reductases | Arabidopsis thaliana |
malfunction | mutants defective in pheophorbide a oxygenase or red chlorophyll catabolite reductase, e.g. acd2 mutants that exhibit a light-dependent cell death phenotype with spontaneous spreading lesions, the mutants develop a lesion mimic phenotype, due to accumulation of breakdown intermediates | Arabidopsis thaliana |
metabolism | the three chl catabolic enzymes, chlorophyllase, pheophorbide a oxygenase (PAO), and red chlorophyll catabolite reductase (RCCR) catalyze chlorophyll breakdown, which is very important for plant development and survival. Chlorophyll breakdown is a prerequisite to detoxify the potentially phototoxic pigment within the vacuoles in order to permit the remobilization of nitrogen from chlorophyll-binding proteins to proceed during senescence. Enzyme RCCR might be required to mediate an efficient interaction between red chlorophyll catabolite (still bound to PAO) and ferredoxin, thereby enabling a fast, regio-, and stereoselective reduction to primary fluorescent chlorophyll catabolite | Arabidopsis thaliana |