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Literature summary for 1.3.7.4 extracted from
- Purification and biochemical properties of phytochromobilin synthase from etiolated oat seedlings (2001), Plant Physiol., 126, 1546-1554.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | sub-micromolar Km for biliverdin IXa | Avena sativa | |
| 0.0012 | - |
reduced ferredoxin | Porphyra umbilicalis ferredoxin | Avena sativa |  |
| 0.0035 | - |
reduced ferredoxin | Spinacia oleracea ferredoxin | Avena sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| etioplast | - |
Avena sativa | 9513 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE | Avena sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | - |
oat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 50000fold | Avena sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Avena sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3Z)-phytochromobilin + oxidized ferredoxin | - |
Avena sativa | biliverdin IXa + reduced ferredoxin | - |
? | |
| biliverdin IXalpha + reduced ferredoxin | - |
Avena sativa | (3Z)-phytochromobilin + oxidized ferredoxin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Avena sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Avena sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.67 | - |
biliverdin IXa | above | Avena sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Avena sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | 2Fe-2S | Avena sativa |
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Release 2023.1 (January 2023)
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