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Literature summary for 1.3.7.7 extracted from
- Characterization of three genes encoding the subunits of light-independent protochlorophyllide reductase in Chlorella protothecoides CS-41 (2006), Biotechnol. Prog., 22, 1050-1055.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)Lys cells | Auxenochlorella protothecoides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Auxenochlorella protothecoides | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
| 49000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
| 58000 | - |
1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyllide a + reduced ferredoxin + ATP | Auxenochlorella protothecoides | - |
protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Auxenochlorella protothecoides | Q6VQA8 and Q6VQA9 and Q7YKW4 | subunit B, subunit L, and subunit N; strain CS-41, also named Auxenochlorella protothecoides strain CS-41 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorophyllide a + reduced ferredoxin + ATP | - |
Auxenochlorella protothecoides | protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 38000 + 1 * 49000 + 1 * 58000, SDS-PAGE | Auxenochlorella protothecoides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChlB | subunit B | Auxenochlorella protothecoides |
| ChlL | subunit L | Auxenochlorella protothecoides |
| ChlN | subunit N | Auxenochlorella protothecoides |
| light-independent protochlorophyllide oxidoreductase | - |
Auxenochlorella protothecoides |
| light-independent protochlorophyllide reductase | - |
Auxenochlorella protothecoides |
| LIPOR | - |
Auxenochlorella protothecoides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4Fe-4S-center | the enzyme contains an Fe-S cluster as redox center in all subunits | Auxenochlorella protothecoides | |
| ATP | subunit L contains the ATP-binding motif | Auxenochlorella protothecoides | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Auxenochlorella protothecoides | the level of theLIPOR subunit L mRNA of Chlorella protothecoides strain CS-41 is much higher in the light than in the dark | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | light-independent protochlorophyllide reductase is required for protochlorophyllide reduction in the dark | Auxenochlorella protothecoides |
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