Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(methylenecyclopropyl)acetyl-CoA | completely inhibits at 0.01 mM | Rattus norvegicus | |
3-methylcrotonyl-CoA | - |
Rattus norvegicus | |
Ag+ | completely inhibits at 0.01 mM | Rattus norvegicus | |
Cu2+ | completely inhibits at 0.01 mM | Rattus norvegicus | |
Hg2+ | completely inhibits at 0.1 mM | Rattus norvegicus | |
Methylmercury iodide | completely inhibits at a concentration of 0.1 mM | Rattus norvegicus | |
N-ethylmaleimide | completely inhibits at a concentration of 2 mM | Rattus norvegicus | |
p-hydroxymercuribenzoate | completely inhibits at a concentration of 0.01 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | 0.4 | n-valeryl-CoA | - |
Rattus norvegicus | |
0.033 | - |
isovaleryl-CoA | with phenazine methosulfate as an artificial electron acceptor | Rattus norvegicus | |
0.4 | - |
n-valeryl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Rattus norvegicus |
175000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | Rattus norvegicus | electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate | 3-methylcrotonyl-CoA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using sonication, ammonium sulfate treatment and column chromatography on DEAE-Sephadex A-50, hydroxylapatite, Matrex Gel Blue A and BioGel A-0.5 m | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.086 | - |
substrate: n-butyryl-CoA | Rattus norvegicus |
0.832 | - |
substrate: n-valeryl-CoA | Rattus norvegicus |
2.68 | - |
substrate: isovaleryl-CoA | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | isovaleryl-CoA is the preferred substrate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
n-valeryl-CoA + acceptor | - |
Rattus norvegicus | pent-2-enoyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isovaleryl-CoA + phenazine methosulfate | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains FAD as a prosthetic group, 0.6 mol FAD per mol of subunit | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
3-methylcrotonyl-CoA | - |
Rattus norvegicus |