Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(methylenecyclopropyl)acetyl-CoA | at 0.001 mM 21% activity remains; at 0.01 mM 3% activity remains | Homo sapiens | |
iodoacetamide | at 2 mM 19% activity remains | Homo sapiens | |
Methylmercury iodide | - |
Homo sapiens | |
N-ethylmaleimide | at 2 mM 64% activity remains | Homo sapiens | |
p-chloromercuribenzoate | at 0.1 mM 18% activity remains | Homo sapiens | |
p-hydroxymercuribenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
electron-transfer flavoprotein | - |
Homo sapiens | |
0.014 | - |
isovaleryl-CoA | - |
Homo sapiens | |
0.0167 | - |
valeryl-CoA | - |
Homo sapiens | |
0.0167 | 0.4 | n-valeryl-CoA | - |
Homo sapiens | |
0.02 | - |
hexanoyl-CoA | - |
Homo sapiens | |
0.025 | - |
butyryl-CoA | - |
Homo sapiens | |
0.833 | - |
phenazine methosulfate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Homo sapiens |
172000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | Homo sapiens | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
propionyl-CoA as substrate and phenazine methosulfate as acceptor or hexanoyl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
0.03 | - |
butyryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
0.09 | - |
valeryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
0.17 | - |
S-2-methylbutyryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
0.51 | - |
butyryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
0.53 | - |
isovaleryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor | Homo sapiens |
0.64 | - |
hexanoyl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
0.72 | - |
isovaleryl-CoA as substrate and 0.012 mM electron transfer flavoprotein as acceptor | Homo sapiens |
1.09 | - |
valeryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
2.91 | - |
isovaleryl-CoA as substrate and phenazine methosulfate as acceptor | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + acceptor | acceptor: phenazine methosulfate | Homo sapiens | but-2-enoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + acceptor | acceptor: phenazine methosulfate | Homo sapiens | hex-2-enoyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + electron-transfer flavoprotein | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein | - |
r | |
isovaleryl-CoA + phenazine methosulfate | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
isovaleryl-CoA + phenazine methosulfate | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol of FAD per subunit | Homo sapiens |