Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and comparison, expression in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E254G | site-directed mutagenesis, inactive mutant | Rattus norvegicus |
E254G/G375E | site-directed mutagenesis, shows no activity with (S)-2-methylbutyryl-CoA in contrast to the wild-type enzyme, reduced activity compared to the wild-type enzyme | Rattus norvegicus |
G375E | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
(S)-2-methylbutyryl-CoA | wild-type enzyme, pH 7.4, 37°C | Rattus norvegicus | |
0.0029 | - |
isovaleryl-CoA | wild-type enzyme, pH 7.4, 37°C | Rattus norvegicus | |
0.0031 | - |
isovaleryl-CoA | mutabt IVD E254G/G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.0035 | - |
(S)-2-methylbutyryl-CoA | mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.0038 | - |
isovaleryl-CoA | mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + electron-transfer flavoprotein | Rattus norvegicus | - |
3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
additional information | Rattus norvegicus | the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P12007 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-methylbutyryl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-methylcrotonyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
butyryl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | crotonyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
hexanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | hex-2-enoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
isovaleryl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
additional information | the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives | Rattus norvegicus | ? | - |
? | |
additional information | broader substrate specificity, overview. The enzyme is also active with octanoyl-CoA, decanoyl-CoA, and dodecanoyl-CoA. No activity with isobutyryl-CoA and myristoyl-CoA, with DCIPIP and phenazine methosulfate as second electron acceptors | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IVD | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
isovaleryl-CoA | mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.3 | - |
(S)-2-methylbutyryl-CoA | wild-type enzyme and mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.32 | - |
isovaleryl-CoA | mutabt IVD E254G/G375E, pH 7.4, 37°C | Rattus norvegicus | |
1.5 | - |
isovaleryl-CoA | wild-type enzyme, pH 7.4, 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
electron transferring flavoprotein | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to acyl-CoA dehydrogenase (ACD) family | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
isovaleryl-CoA | mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.086 | - |
(S)-2-methylbutyryl-CoA | mutant IVD G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.1 | - |
isovaleryl-CoA | mutabt IVD E254G/G375E, pH 7.4, 37°C | Rattus norvegicus | |
0.16 | - |
(S)-2-methylbutyryl-CoA | wild-type enzyme, pH 7.4, 37°C | Rattus norvegicus | |
0.53 | - |
isovaleryl-CoA | wild-type enzyme, pH 7.4, 37°C | Rattus norvegicus |