Protein Variants | Comment | Organism |
---|---|---|
T255E | same activity with octanoyl-CoA as wild-type, activity/chain length profile is, however, narrower | Homo sapiens |
T255E/E376G | chimeric medium-long chain acyl-CoA dehydrogenase, has 20% of the activity of medium-chain acyl-CoA dehydrogenase and 25% that of long-chain acyl-CoA dehydrogenase activity with its best substrates octanoyl-CoA and dodecanoyl-CoA respectively, activity maximum is shifted to C12 and C14-CoA, enzyme shows an enhanced rate of reoxidation with oxygen | Homo sapiens |
T255E/E376T | shifts the chain length activity profile to higher values, drastic reduction of maximal velocity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
octanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.0013 | - |
hexadecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.0014 | - |
hexanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
0.0016 | - |
hexadecanoyl-CoA | - |
Homo sapiens | |
0.0023 | - |
tetradecanoyl-CoA | - |
Homo sapiens | |
0.0025 | - |
decanoyl-CoA | - |
Homo sapiens | |
0.0025 | - |
dodecanoyl-CoA | - |
Homo sapiens | |
0.0034 | - |
octanoyl-CoA | - |
Homo sapiens | |
0.0042 | - |
decanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.0055 | - |
tetradecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.0066 | - |
dodecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.008 | - |
octanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
0.01 | - |
decanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
0.015 | - |
hexanoyl-CoA | - |
Homo sapiens | |
0.05 | - |
decanoyl-CoA | T255E/E376T double mutant enzyme | Homo sapiens | |
0.05 | - |
dodecanoyl-CoA | T255E/E376T double mutant enzyme | Homo sapiens | |
0.07 | - |
butanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
0.175 | - |
butanoyl-CoA | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, T255E, T255E/E367G and T255E/E367T mutant enzymes | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein | the catalytic base responsible for the alpha-proton abstraction is E376 in medium-chain acyl-CoA dehydrogenase, while that in long-chain acyl-CoA dehydrogenase is E255 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + acceptor | - |
Homo sapiens | crotonyl-CoA + reduced acceptor | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.167 | - |
octanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.233 | - |
decanoyl-CoA | T255E/E376T double mutant enzyme | Homo sapiens | |
0.333 | - |
decanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
0.617 | - |
hexadecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
2.33 | - |
hexadecanoyl-CoA | - |
Homo sapiens | |
2.67 | - |
tetradecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
2.83 | - |
hexanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
3.33 | - |
dodecanoyl-CoA | T255E/E376G double mutant enzyme | Homo sapiens | |
4.67 | - |
tetradecanoyl-CoA | - |
Homo sapiens | |
8.33 | - |
butanoyl-CoA | wild-type and T255E mutant enzyme | Homo sapiens | |
9.17 | - |
dodecanoyl-CoA | - |
Homo sapiens | |
9.67 | - |
decanoyl-CoA | - |
Homo sapiens | |
11.3 | - |
decanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
13.2 | - |
octanoyl-CoA | T255E mutant enzyme | Homo sapiens | |
16.3 | - |
octanoyl-CoA | - |
Homo sapiens | |
16.8 | - |
hexanoyl-CoA | - |
Homo sapiens |