Cloned (Comment) | Organism |
---|---|
transformation in Escherichia coli for detection of pyrimidine prototrophic colonies with a resistance of ampicillin | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
hanging drop vapor diffusion technique, 30% polyethylene glycol, 0.2 M sodium acetate, 0.1 M Tris-HCl, pH 8.5, monoclinic crystals, space group P21 | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Lactococcus lactis |
66000 | - |
gel filtration | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | Lactococcus lactis | fourth step in UMP-biosynthesis | orotate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis | - |
only enzyme A | - |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
streptomycin precipitation, ion-exchange, hydroxylapatite | Lactococcus lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-dihydroorotate + fumarate = orotate + succinate | ping-pong mechanism | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17 | - |
- |
Lactococcus lactis |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, several months, without loss of activity | Lactococcus lactis |
-20°C, 50% glycerol, stable for several months | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | 2,6-dichlorophenolindophenol, potassium hexacyanoferrate(III) and molecular oxygen used as electron acceptors | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + acceptor | fourth step in UMP-biosynthesis | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptor: 2,6-dichlorophenolindophenol | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | not acceptors: NAD+, NADP+, coenzymes Q6, Q10, K2 | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | no substrate: dihydrouracil | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
dihydroorotate + acceptor | acceptors: potassium hexacyanoferrate(III), to a lower extent molecular oxygen | Lactococcus lactis | orotate + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Lactococcus lactis |
dimer | 2 * 34000-36000, SDS-PAGE | Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, loss of half of the activity | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lactococcus lactis |
7.5 | 9 | - |
Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | assay in this range | Lactococcus lactis |
7.5 | 9 | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one molecule per subunit | Lactococcus lactis |