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Literature summary for 1.3.98.1 extracted from
- A new type of dihydroorotate dehydrogenase, type 1S, from the thermoacidophilic archaeon Sulfolobus solfataricus (2002), Extremophiles, 6, 245-251.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Orotate | competitive product inhibition | Saccharolobus solfataricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0442 | - |
dihydroorotate | pH 8.5, 70°C | Saccharolobus solfataricus | |
| 0.344 | - |
ferricyanide | pH 8.5, 70°C | Saccharolobus solfataricus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharolobus solfataricus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
enzyme type 1S | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharolobus solfataricus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (S)-dihydroorotate + fumarate = orotate + succinate | two-site ping-pong mechanism, catalytic base is serine | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3948 | - |
pH 8.5, 70°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroorotate + 2,6-dichlorophenolindophenol | - |
Saccharolobus solfataricus | orotate + ? | - |
? | |
| dihydroorotate + ferricyanide | - |
Saccharolobus solfataricus | orotate + ferrocyanide | - |
? | |
| dihydroorotate + O2 | - |
Saccharolobus solfataricus | orotate + H2O2 | - |
? | |
| additional information | no acceptor: fumarate, NAD+, coenzymes ubiquinone-6, ubiquinone-10 | Saccharolobus solfataricus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heteromer | x * catalytic subunit, 31100, + x * electron acceptor subunit, 23600, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ferricyanide | - |
Saccharolobus solfataricus | |
| additional information | no cofactor: NAD+, coenzymes ubiquinone-6, ubiquinone-10 | Saccharolobus solfataricus | |
| ubiquinone-0 | - |
Saccharolobus solfataricus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0237 | - |
Orotate | pH 8.5, 70°C | Saccharolobus solfataricus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | isoelecric focusing | - |
6 |
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