Crystallization (Comment) | Organism |
---|---|
wild-type enzyme and mutants N67A, K213E, P56A, R57A, K136E | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
K136E | little changes in activity | Lactococcus lactis |
K213E | decrease in activity against dichlorophenolindophenol | Lactococcus lactis |
N127A | reduced activity | Lactococcus lactis |
N193A | drastically reduced activiy | Lactococcus lactis |
N67A | drastically reduced activiy | Lactococcus lactis |
P131A | decrease in activtiy against fumarate | Lactococcus lactis |
P56A | drastically reduced activiy | Lactococcus lactis |
R50E | little changes in activity | Lactococcus lactis |
R57A | increase in activity | Lactococcus lactis |
S129A | decrease in activtiy against fumarate | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
class 1A enzyme | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
pH 8.0, 25°C, mutant N67A, substrate O2 | Lactococcus lactis |
0.11 | - |
pH 8.0, 25°C, mutant N67A, substrate dichlorophenolindophenol | Lactococcus lactis |
0.14 | - |
pH 8.0, 25°C, mutant N193A, substrate fumarate | Lactococcus lactis |
0.15 | - |
pH 8.0, 25°C, mutant N193A, substrate dichlorophenolindophenol | Lactococcus lactis |
0.15 | - |
pH 8.0, 25°C, mutant P56A, substrate O2 | Lactococcus lactis |
0.17 | - |
pH 8.0, 25°C, mutant N193A, substrate O2 | Lactococcus lactis |
0.3 | - |
pH 8.0, 25°C, mutant N67A, substrate fumarate | Lactococcus lactis |
0.47 | - |
pH 8.0, 25°C, mutant P56A, substrate dichlorophenolindophenol | Lactococcus lactis |
0.48 | - |
pH 8.0, 25°C, mutant P56A, substrate fumarate | Lactococcus lactis |
0.75 | - |
pH 8.0, 25°C, mutant N127A, substrate dichlorophenolindophenol | Lactococcus lactis |
1 | - |
pH 8.0, 25°C, mutant N127A, substrate O2 | Lactococcus lactis |
1.2 | - |
pH 8.0, 25°C, mutant N127A, substrate fumarate | Lactococcus lactis |
1.6 | - |
pH 8.0, 25°C, mutant K136E, substrate O2 | Lactococcus lactis |
1.8 | - |
pH 8.0, 25°C, mutant S129A, substrate O2 | Lactococcus lactis |
1.9 | - |
pH 8.0, 25°C, mutant K213E, substrate O2 | Lactococcus lactis |
2 | - |
pH 8.0, 25°C, wild-type, substrate O2 | Lactococcus lactis |
2.3 | - |
pH 8.0, 25°C, mutant P131A, substrate O2 | Lactococcus lactis |
2.4 | - |
pH 8.0, 25°C, mutant K213E, substrate dichlorophenolindophenol | Lactococcus lactis |
2.8 | - |
pH 8.0, 25°C, mutant S129A, substrate fumarate | Lactococcus lactis |
3.3 | - |
pH 8.0, 25°C, mutant R50E, substrate O2 | Lactococcus lactis |
3.3 | - |
pH 8.0, 25°C, mutant R57A, substrate O2 | Lactococcus lactis |
3.7 | - |
pH 8.0, 25°C, mutant P131A, substrate fumarate | Lactococcus lactis |
4 | - |
pH 8.0, 25°C, mutant P131A, substrate dichlorophenolindophenol | Lactococcus lactis |
7.2 | - |
pH 8.0, 25°C, mutant K213E, substrate fumarate | Lactococcus lactis |
7.2 | - |
pH 8.0, 25°C, mutant R50E, substrate dichlorophenolindophenol | Lactococcus lactis |
11.7 | - |
pH 8.0, 25°C, mutant S129A, substrate dichlorophenolindophenol | Lactococcus lactis |
13 | - |
pH 8.0, 25°C, mutant K136E, substrate dichlorophenolindophenol | Lactococcus lactis |
13.6 | - |
pH 8.0, 25°C, wild-type, substrate dichlorophenolindophenol | Lactococcus lactis |
15.8 | - |
pH 8.0, 25°C, wild-type, substrate fumarate | Lactococcus lactis |
18.1 | - |
pH 8.0, 25°C, mutant K136E, substrate fumarate | Lactococcus lactis |
19 | - |
pH 8.0, 25°C, mutant R50E, substrate fumarate | Lactococcus lactis |
27 | - |
pH 8.0, 25°C, mutant R57A, substrate fumarate | Lactococcus lactis |
65.7 | - |
pH 8.0, 25°C, mutant R57A, substrate dichlorophenolindophenol | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + 2,6-dichlorophenolindophenol | - |
Lactococcus lactis | orotate + reduced 2,6-dichlorophenolindophenol | - |
? | |
dihydroorotate + fumarate | - |
Lactococcus lactis | orotate + ? | - |
? | |
dihydroorotate + O2 | - |
Lactococcus lactis | orotate + H2O2 | - |
? |