Protein Variants | Comment | Organism |
---|---|---|
H185A | 4fold increase in KM-value of CoQD, 50% increase in KM-value of L-dihydroorotate | Plasmodium falciparum |
R265A | 2fold increase in KM-value of CoQD, 15% increase in KM-value of L-dihydroorotate | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(1-naphthyl)-3-[2-(trifluoromethyl)phenyl]urea | 50% inhibition at 0.0004 mM, wild type, 0.00034 mM, mutant R265A, above 0.2, mutant H185A | Plasmodium falciparum | |
1-(2-methoxyphenyl)-3-(1-naphthyl)urea | 50% inhibition at 0.00023 mM, wild type, 0.00024 mM, mutant R265A, 0.140, mutant H185A | Plasmodium falciparum | |
1-(4-chlorophenyl)-3-quinolin-8-ylurea | 50% inhibition at 0.00078 mM, wild type, 0.002 mM, mutant R265A, above 0.4, mutant H185A | Plasmodium falciparum | |
2-methyl-3-nitro-N-[3-(trifluoromethyl)phenyl]benzamide | 50% inhibition at 0.00008 mM, wild type, 0.00037 mM, mutant R265A, 0.120, mutant H185A | Plasmodium falciparum | |
2-methyl-N-1-naphthyl-3-nitrobenzamide | 50% inhibition at 0.00008 mM, wild type, 0.00048 mM, mutant R265A, 0.180, mutant H185A | Plasmodium falciparum | |
N-(2,4-dichlorophenyl)-2-naphthamide | 50% inhibition at 0.00005 mM, wild type, 0.00008 mM, mutant R265A, above 0.05, mutant H185A | Plasmodium falciparum | |
N-(2-fluorophenyl)naphthalene-2-carboxamide | 50% inhibition at 0.00047 mM, wild type, 0.00030 mM, mutant R265A, 0.21, mutant H185A | Plasmodium falciparum | |
N-(3,4-dichlorophenyl)-2-methyl-3-nitrobenzamide | 50% inhibition at 0.00008 mM, wild type, 0.0028 mM, mutant R265A, 0.06, mutant H185A | Plasmodium falciparum | |
N-(3,4-difluorophenyl)-2-methyl-3-nitrobenzamide | 50% inhibition at 0.00026 mM, wild type, 0.00082 mM, mutant R265A, 0.25, mutant H185A | Plasmodium falciparum | |
N-(3,5-dichlorophenyl)-2-methyl-3-nitrobenzamide | 50% inhibition at 0.00002 mM, wild type, 0.0004 mM, mutant R265A, 0.13, mutant H185A | Plasmodium falciparum | |
N-(3-bromophenyl)-2-methyl-3-nitrobenzamide | 50% inhibition at 0.00006 mM, wild type, 0.0012 mM, mutant R265A, 0.140, mutant H185A | Plasmodium falciparum | |
N-(3-chloro-4-fluorophenyl)-2-methyl-3-nitrobenzamide | 50% inhibition at 0.0001 mM, wild type, 0.0005 mM, mutant R265A, 0.13, mutant H185A | Plasmodium falciparum | |
N-(4-bromo-2-methylphenyl)-2-naphthamide | 50% inhibition at 0.00005 mM, wild type, 0.00005 mM, mutant R265A, 0.57, mutant H185A | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
CoQ | pH 8.0, 22°C, wild-type | Plasmodium falciparum | |
0.032 | - |
CoQ | pH 8.0, 22°C, mutant R265A | Plasmodium falciparum | |
0.066 | - |
CoQ | pH 8.0, 22°C, mutant H185A | Plasmodium falciparum | |
0.091 | - |
L-dihydroorotate | pH 8.0, 22°C, wild-type | Plasmodium falciparum | |
0.105 | - |
L-dihydroorotate | pH 8.0, 22°C, mutant R265A | Plasmodium falciparum | |
0.123 | - |
L-dihydroorotate | pH 8.0, 22°C, mutant H185A | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihydroorotate + 2,6-dichloroindophenol | - |
Plasmodium falciparum | orotate + ? | - |
? | |
L-dihydroorotate + CoQ | - |
Plasmodium falciparum | orotate + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
L-dihydroorotate | pH 8.0, 22°C, mutant H185A | Plasmodium falciparum | |
4.6 | - |
L-dihydroorotate | pH 8.0, 22°C, mutant R265A | Plasmodium falciparum | |
7.8 | - |
L-dihydroorotate | pH 8.0, 22°C, wild-type | Plasmodium falciparum |