Literature summary for 1.3.98.1 extracted from

Shi, J.; Dertouzos, J.; Gafni, A.; Steel, D.; Palfey, B.A.
Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis (2006), Proc. Natl. Acad. Sci. USA, 103, 5775-5780.

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Application

Application	Comment	Organism
additional information	significant heterogeneity in the catalytic behaviors of individual dimer molecules, very similar reaction rates in both the reductive and oxidative half-reactions for different DHODA dimers, single-molecule data provide strong evidence for half-sites reactivity, in which only one subunit reacts at a time	Lactococcus lactis

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain SØ6645	Lactococcus lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by ion-exchange chromatography	Lactococcus lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dihydroorotate + fumarate	-	Lactococcus lactis	orotate + reduced fumarate	-	?

Synonyms

Synonyms	Comment	Organism
DHODA	-	Lactococcus lactis

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Release 2023.1 (January 2023)