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Literature summary for 1.3.99.18 extracted from

  • Sauter, M.; Tshisuaka, B.; Fetzner, S.; Lingens, F.
    XX. Quinaldic acid 4-oxidoreductase from Pseudomonas sp. AK-2 compared to other procaryotic molybdenum-containing hydroxylases (1993), Biol. Chem. Hoppe-Seyler, 374, 1037-1046.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Acriflavine
-
Pseudomonas sp.
CoCl2
-
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
KCN 74% reactivation with S2- Pseudomonas sp.
methanol
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
NaAsO2
-
Pseudomonas sp.
NiCl2
-
Pseudomonas sp.
p-hydroxymercuribenzoate
-
Pseudomonas sp.
Quinacrine
-
Pseudomonas sp.
ZnSO4
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
Quinaldate with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate Pseudomonas sp.
1.6
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride with quinaldate as cosubstrate Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme Pseudomonas sp.
Molybdenum enzyme contains 1.6 g-atom per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE Pseudomonas sp.
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE Pseudomonas sp.
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE Pseudomonas sp.
300000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
AK-2
-
Pseudomonas sp. AK-2
-
AK-2
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.82
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
Pseudomonas sp. kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
-
Pseudomonas sp. AK-2 kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
r
quinaldate + acceptor acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid Pseudomonas sp. kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinaldate + acceptor i.e. quinoline 2-carboxylic acid Pseudomonas sp. kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinaldate + acceptor acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride Pseudomonas sp. kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinaldate + acceptor acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid Pseudomonas sp. AK-2 kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinaldate + acceptor i.e. quinoline 2-carboxylic acid Pseudomonas sp. AK-2 kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinaldate + acceptor acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride Pseudomonas sp. AK-2 kynurenate + reduced acceptor i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate ?
quinoline 8-carboxylic acid + reduced acceptor
-
Pseudomonas sp. ?
-
?
quinoline 8-carboxylic acid + reduced acceptor
-
Pseudomonas sp. AK-2 ?
-
?

Subunits

Subunits Comment Organism
hexamer 2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
above 60°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
5 min, 32% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 1.8 mol of FAD per mol of enzyme Pseudomonas sp.
Pterin molybdenum cofactor molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme Pseudomonas sp.