Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diphenylamine | - |
Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0166 | - |
all-trans-zeta-carotene | pH 8.0, 30°C | Rhodobacter capsulatus | |
0.0333 | - |
15-cis-phytoene | pH 8.0, 30°C | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 3 acceptor | Rhodobacter capsulatus | involved in carotenoid biosynthesis | all-trans-neurosporene + 3 reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
15-cis-phytoene + 3 acceptor = all-trans-neurosporene + 3 reduced acceptor | overall reaction | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00029 | - |
pH 8.0, 30°C | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-epoxyphytoene + acceptor | - |
Rhodobacter capsulatus | 1,2-epoxyneurosporene + reduced acceptor | - |
? | |
15-cis-phytoene + 3 acceptor | involved in carotenoid biosynthesis | Rhodobacter capsulatus | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
15-cis-phytoene + 3 acceptor | intermediates are phytofluene and zeta-carotene. The proportion of all-trans versus cis isomers is about 20% for phytofluene, 46% for zeta-carotene, and 72% for neurosporene | Rhodobacter capsulatus | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
all-trans-phytofluene + 2 acceptor | - |
Rhodobacter capsulatus | all-trans-neurosporene + 2 reduced acceptor | - |
? | |
all-trans-zeta-carotene + acceptor | - |
Rhodobacter capsulatus | all-trans-neurosporene + reduced acceptor | - |
? | |
additional information | C30-diapophytoene is not a substrate | Rhodobacter capsulatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
3-step phytoene desaturase | - |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a low basal reaction is found in the absence of all coenzymes. Upon addition of FAD the activity is stimulated 5-fold. Km-value: 0.0049 mM | Rhodobacter capsulatus | |
additional information | NAD+, NADP+ or ATP have no positive effect | Rhodobacter capsulatus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.23 | - |
pH 8.0, 30°C | Rhodobacter capsulatus | Diphenylamine |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in carotenoid biosynthesis | Rhodobacter capsulatus |