Cloned (Comment) | Organism |
---|---|
screening of a cosmid gene library, DNA and amino acid sequence determination and analysis, expression of His6-tagged GDHDes in Escherichia coli strain BL21(DE3) | Desulfococcus multivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutaconyl-CoA | product inhibition | Desulfococcus multivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
glutaryl-CoA | pH 7.8, 30°C | Desulfococcus multivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Desulfococcus multivorans | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | Desulfococcus multivorans | - |
glutaconyl-CoA + FADH2 | - |
? | |
additional information | Desulfococcus multivorans | GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfococcus multivorans | C3UVB0 | a sulfate reducing organism | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged GDHDes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Desulfococcus multivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | - |
Desulfococcus multivorans | glutaconyl-CoA + FADH2 | - |
? | |
additional information | GDHGeo contains highly conserved amino acid residues that are specifically involved in the decarboxylation of the intermediate glutaconyl-CoA | Desulfococcus multivorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000-44000 | Desulfococcus multivorans |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Desulfococcus multivorans |
GDHDes | - |
Desulfococcus multivorans |
nondecarboxylating glutaryl-coenzyme A dehydrogenase | - |
Desulfococcus multivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Desulfococcus multivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Desulfococcus multivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | GDHDes contains 0.6-0.7 FAD/monomer | Desulfococcus multivorans |