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Literature summary for 1.3.99.4 extracted from

  • Choi, K.P.; Molnar, I.; Yamashita, M.; Murooka, Y.
    Purification and characterization of the 3-ketosteroid-DELTA1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans (1995), J. Biochem., 117, 1043-1049.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Pimelobacter simplex
CuCl2
-
Pimelobacter simplex
HgCl2
-
Pimelobacter simplex
additional information not sensitive to carbonyl reagents and little sensitivity to metal chelating agents Pimelobacter simplex
p-chloromercuribenzoate
-
Pimelobacter simplex

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
4-androstene-3,17-dione
-
Pimelobacter simplex

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
SDS-PAGE, gel filtration Pimelobacter simplex

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 10%, stabilization Pimelobacter simplex

Organism

Organism UniProt Comment Textmining
Pimelobacter simplex
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pimelobacter simplex

Storage Stability

Storage Stability Organism
4°C, 10% glycerol, at least 3 months stable Pimelobacter simplex

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,11,17-trione + acceptor
-
Pimelobacter simplex 1,4-androstene-3,11,17-trione + reduced acceptor
-
?
4-androstene-3,17-dione + acceptor
-
Pimelobacter simplex 1,4-androstene-3,17-dione + reduced acceptor
-
?
4-pregnene-3,20-dione + acceptor
-
Pimelobacter simplex 1,4-pregnene-3,20 dione + reduced acceptor
-
?
cortisone + acceptor
-
Pimelobacter simplex 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
?
testosterone + acceptor
-
Pimelobacter simplex 17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE, PAGE, gel filtration, N-terminal sequence Pimelobacter simplex

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pimelobacter simplex

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 maximum at 40°C, enzyme stable below 40°C Pimelobacter simplex

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
substrate 4-androstene-3,17-dione, buffer 0.1 M glycine-NaOH Pimelobacter simplex