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Literature summary for 1.3.99.4 extracted from
- 3-Ketosteroid-DELTA1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme (1998), J. Biochem., 124, 1026-1032.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rhodococcus rhodochrous |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
4-androstene-3,17-dione | - |
Rhodococcus rhodochrous |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | partly, recombinant protein in Escherichia coli | Rhodococcus rhodochrous | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE, recombinant protein, expression in Escherichia coli | Rhodococcus rhodochrous |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus rhodochrous | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli | Rhodococcus rhodochrous |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + phenazine methosulfate | - |
Rhodococcus rhodochrous | 1,4-androstadiene-3,17-dione + reduced phenazine methosulfate | - |
r | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | absorption maxima at 276, 362, 458 nm | Rhodococcus rhodochrous | |
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