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Literature summary for 1.4.1.1 extracted from

  • Honorat, A.; Monot, F.; Ballerini, D.
    Synthesis of L-alanine and L-valine by enzyme systems from Bacillus megaterium (1990), Enzyme Microb. Technol., 12, 515-520.
No PubMed abstract available
Search for more literature for 1.4.1.1

Inhibitors

Inhibitors Comment Organism Structure
CoCl2
-
 Priestia megaterium
CuSO4
-
 Priestia megaterium
HgCl2
-
 Priestia megaterium
pyruvate substrate inhibition Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NADH
-
 Priestia megaterium
0.55
-
 pyruvate
-
 Priestia megaterium
235
-
 NH4+
-
 Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Priestia megaterium
11.2
-
-
 Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH 11.7% of the activity with pyruvate Priestia megaterium L-Gln + NAD+ + H2O
-
 ?
3-bromopyruvate + NH3 + NADH 5.3% of the activity with pyruvate Priestia megaterium 2-amino-3-bromopropanoate + NAD+ + H2O
-
 ?
4-methyl-2-oxopentanoate + NH3 + NADH 1.6% of the activity with pyruvate Priestia megaterium 2-amino-4-methylpentanoate + NAD+ + H2O
-
 ?
D-Ala + H2O + NAD+ 32.3% of the activity with L-Ala Priestia megaterium pyruvate + NH3 + NADH
-
 ?
D-Ser + H2O + NAD+ 1.3% of the activity with L-Ser Priestia megaterium 3-hydroxypyruvate + NH3 + NADH
-
 ?
glyoxylate + NH3 + NADH 6.2% of the activity with pyruvate Priestia megaterium aminoacetate + NAD+ + H2O
-
 ?
L-Ala + H2O + NAD+
-
 Priestia megaterium pyruvate + NH3 + NADH
-
 r
L-Ser + H2O + NAD+ 7.9% of the activity with L-Ala Priestia megaterium 3-hydroxypyruvate + NH3 + NADH
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.5, half-life: 22 days Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 reductive amination Priestia megaterium
10.5
-
 deamination of L-Ala Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Priestia megaterium
NADH
-
 Priestia megaterium