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Literature summary for 1.4.1.1 extracted from
- Alanine dehydrogenases from two Bacillus species with distinct thermostabilities: molecular cloning, DNA and protein sequence determination, and structural comparison with other NAD(P)(+)-dependent dehydrogenases (1990), Biochemistry, 29, 1009-1015.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Escherichia coli | Lysinibacillus sphaericus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39465 | - |
6 * 39465, calculation from nucleotide sequence | Lysinibacillus sphaericus |
| 39500 | - |
6 * 39500, recombinant enzyme, SDS-PAGE | Lysinibacillus sphaericus |
| 235000 | - |
recombinant enzyme, gel filtration | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Lysinibacillus sphaericus | - |
gene cloned and expressed in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala + H2O + NAD+ | - |
Geobacillus stearothermophilus | pyruvate + NH3 + NADH | - |
r |  |
| L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Geobacillus stearothermophilus |
| hexamer | 6 * 39500, recombinant enzyme, SDS-PAGE | Lysinibacillus sphaericus |
| hexamer | 6 * 39465, calculation from nucleotide sequence | Lysinibacillus sphaericus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
5 min, 50% loss of activity | Lysinibacillus sphaericus |
| 85 | - |
5 min, 50% loss of activity | Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Geobacillus stearothermophilus | |
| NAD+ | - |
Lysinibacillus sphaericus | |
| NADH | - |
Geobacillus stearothermophilus | |
| NADH | - |
Lysinibacillus sphaericus | |
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