Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | - |
Streptomyces fradiae | |
D-Ala | - |
Streptomyces fradiae | |
HgCl2 | - |
Streptomyces fradiae | |
L-Ala | - |
Streptomyces fradiae | |
L-Ser | - |
Streptomyces fradiae | |
MgCl2 | - |
Streptomyces fradiae | |
PCMB | - |
Streptomyces fradiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | - |
Streptomyces fradiae | |
0.18 | - |
NAD+ | - |
Streptomyces fradiae | |
0.23 | - |
pyruvate | - |
Streptomyces fradiae | |
2.4 | - |
2-oxobutanoate | - |
Streptomyces fradiae | |
2.5 | - |
oxaloacetate | - |
Streptomyces fradiae | |
10 | - |
L-Ala | - |
Streptomyces fradiae | |
11.6 | - |
NH4+ | - |
Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, SDS-PAGE | Streptomyces fradiae |
205000 | 210000 | gel filtration, equilibrium ultracentrifugation | Streptomyces fradiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces fradiae |
Storage Stability | Organism |
---|---|
-20°C, 0.2 M Tris/HCl buffer, pH 7.4, 2 months, stable | Streptomyces fradiae |
-4°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h, stable | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH | 15% of maximal activity | Streptomyces fradiae | 2-aminobutanoate + H2O + NAD+ | - |
? | |
L-Ala + H2O + 1,N6-etheno-NAD+ | 59% of the activity with NAD+ | Streptomyces fradiae | pyruvate + NH3 + 1,N6-etheno-NADH | - |
? | |
L-Ala + H2O + 3-acetylpyridine-NAD+ | 62% of the activity with NAD+ | Streptomyces fradiae | pyruvate + H2O + 3-acetylpyridine-NADH | - |
? | |
L-Ala + H2O + deamino-NAD+ | 77% of the activity with NAD+ | Streptomyces fradiae | pyruvate + NH3 + deamino-NADH | - |
? | |
L-Ala + H2O + NAD+ | - |
Streptomyces fradiae | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + nicotinamide guanine dinucleotide | 71% of the activity with NAD+ | Streptomyces fradiae | ? | - |
? | |
oxaloacetate + NH3 + NADH | 94% of maximal activity | Streptomyces fradiae | L-Asp + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, SDS-PAGE | Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reductive amination | Streptomyces fradiae |
10 | - |
oxidative deamination | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,N6-etheno-NAD+ | 59% of the activity with NAD+ | Streptomyces fradiae | |
3-acetylpyridine-NAD+ | 62% of the activity with NAD+ | Streptomyces fradiae | |
deamino-NAD+ | 77% of the activity with NAD+ | Streptomyces fradiae | |
NAD+ | - |
Streptomyces fradiae | |
NADH | - |
Streptomyces fradiae | |
nicotinamide guanine dinucleotide | 71% of the activity with NAD+ | Streptomyces fradiae |