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Literature summary for 1.4.1.1 extracted from

  • Vancura, A.; Vancurova, I.; Volc, J.; Jones, S.K.T.; Flieger, M.; Basarova, G.; Behal, V.
    Alanine dehydrogenase from Streptomyces fradiae. Purification and properties (1989), Eur. J. Biochem., 179, 221-227.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Streptomyces fradiae
D-Ala
-
Streptomyces fradiae
HgCl2
-
Streptomyces fradiae
L-Ala
-
Streptomyces fradiae
L-Ser
-
Streptomyces fradiae
MgCl2
-
Streptomyces fradiae
PCMB
-
Streptomyces fradiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADH
-
Streptomyces fradiae
0.18
-
NAD+
-
Streptomyces fradiae
0.23
-
pyruvate
-
Streptomyces fradiae
2.4
-
2-oxobutanoate
-
Streptomyces fradiae
2.5
-
oxaloacetate
-
Streptomyces fradiae
10
-
L-Ala
-
Streptomyces fradiae
11.6
-
NH4+
-
Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
4 * 51000, SDS-PAGE Streptomyces fradiae
205000 210000 gel filtration, equilibrium ultracentrifugation Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces fradiae

Storage Stability

Storage Stability Organism
-20°C, 0.2 M Tris/HCl buffer, pH 7.4, 2 months, stable Streptomyces fradiae
-4°C, 0.2 M Tris/HCl buffer, pH 7.4, 24 h, stable Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH 15% of maximal activity Streptomyces fradiae 2-aminobutanoate + H2O + NAD+
-
?
L-Ala + H2O + 1,N6-etheno-NAD+ 59% of the activity with NAD+ Streptomyces fradiae pyruvate + NH3 + 1,N6-etheno-NADH
-
?
L-Ala + H2O + 3-acetylpyridine-NAD+ 62% of the activity with NAD+ Streptomyces fradiae pyruvate + H2O + 3-acetylpyridine-NADH
-
?
L-Ala + H2O + deamino-NAD+ 77% of the activity with NAD+ Streptomyces fradiae pyruvate + NH3 + deamino-NADH
-
?
L-Ala + H2O + NAD+
-
Streptomyces fradiae pyruvate + NH3 + NADH
-
r
L-Ala + H2O + nicotinamide guanine dinucleotide 71% of the activity with NAD+ Streptomyces fradiae ?
-
?
oxaloacetate + NH3 + NADH 94% of maximal activity Streptomyces fradiae L-Asp + H2O + NAD+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 51000, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
reductive amination Streptomyces fradiae
10
-
oxidative deamination Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
1,N6-etheno-NAD+ 59% of the activity with NAD+ Streptomyces fradiae
3-acetylpyridine-NAD+ 62% of the activity with NAD+ Streptomyces fradiae
deamino-NAD+ 77% of the activity with NAD+ Streptomyces fradiae
NAD+
-
Streptomyces fradiae
NADH
-
Streptomyces fradiae
nicotinamide guanine dinucleotide 71% of the activity with NAD+ Streptomyces fradiae