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Literature summary for 1.4.1.1 extracted from

  • Kim, E.K.; Fitt, P.S.
    Partial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase (1977), Biochem. J., 161, 313-320.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.1

General Stability

General Stability Organism
absolute requirement of high ionic strength for stability Halobacterium salinarum

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 10 mM, 36% inhibition of reductive amination Halobacterium salinarum
Co2+ 10 mM, 60% inhibition of reductive amination Halobacterium salinarum
Cu2+ 10 mM, 60% inhibition of reductive amination Halobacterium salinarum
D-2-aminobutanoate
-
 Halobacterium salinarum
D-Ala
-
 Halobacterium salinarum
Fe2+ 10 mM, 85% inhibition of reductive amination Halobacterium salinarum
Fe3+ 10 mM, 85% inhibition of reductive amination Halobacterium salinarum
Mn2+ 10 mM, 60% inhibition of reductive amination Halobacterium salinarum
Ni2+ 10 mM, 60% inhibition of reductive amination Halobacterium salinarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 NADH
-
 Halobacterium salinarum
0.5
-
 NAD+
-
 Halobacterium salinarum
0.8
-
 pyruvate
-
 Halobacterium salinarum
7
-
 L-Ala
-
 Halobacterium salinarum

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ Halobacterium salinarum
KCl reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+, absolute requirement for K+ in oxidative deamination, completely inactive with Na+ or NH4+ Halobacterium salinarum
Li+ reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ Halobacterium salinarum
additional information absolute requirement for high ionic strength for optimum activity Halobacterium salinarum
NaCl reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ Halobacterium salinarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72500
-
-
 Halobacterium salinarum

Organism

Organism UniProt Comment Textmining
Halobacterium salinarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Halobacterium salinarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168
-
-
 Halobacterium salinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH no activity Halobacterium salinarum L-Gln + NAD+ + H2O
-
 ?
4-methyl-2-oxopentanoate + NH3 + NADH
-
 Halobacterium salinarum 2-amino-4-methylpentanoate + NAD+ + H2O
-
 ?
glyoxylate + NH3 + NADH
-
 Halobacterium salinarum aminoacetate + NAD+ + H2O
-
 ?
L-2-aminobutanoate + H2O + NAD+
-
 Halobacterium salinarum 2-oxobutanoate + NH3 + NADH + H+
-
 r
L-Ala + H2O + NAD+
-
 Halobacterium salinarum pyruvate + NH3 + NADH
-
 r
oxaloacetate + NH3 + NADH
-
 Halobacterium salinarum L-Asp + H2O + NAD+
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Halobacterium salinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65 reductive amination, in presence of KCl Halobacterium salinarum
65 70 reductive amination in presence of NaCl Halobacterium salinarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 50% of activity maximum, 65°C: optimum, sharp decrease of activity above, no activity at 75°C Halobacterium salinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 8.5, 50 min, 40% loss of activity Halobacterium salinarum
60
-
 pH 8.5, 10 min, about 45% loss of activity Halobacterium salinarum
70
-
 pH 8.5, 10 min, 90% loss of activity Halobacterium salinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 both directions Halobacterium salinarum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Halobacterium salinarum
NADH
-
 Halobacterium salinarum