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Literature summary for 1.4.1.1 extracted from

  • Scandurra, G.M.; Ryan, A.A.; Pinto, R.; Britton, W.J.; Triccas, J.A.
    Contribution of L-alanine dehydrogenase to in vivo persistence and protective efficacy of the BCG vaccine (2006), Microbiol. Immunol., 50, 805-810.
    View publication on PubMed
Search for more literature for 1.4.1.1

Application

Application Comment Organism
medicine L-alanine catabolism can be conferred on Mycobacterium bovis BCG by introduction of the gene encoding Ald of Mycobacterium tuberculosis, restoration of Ald activity does not change the in vivo growth of BCG in macrophages or mice, and protection against aerosol Mycobacterium tuberculosis infection is not altered by addition of the gene encoding Ald to the BCG vaccine Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
into vector pJEX58, resulting plasmid pGS3 expressed in Mycobacterium bovis BCG Pasteur Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 immunoblotting, detectable in sonicates of Mycobacterium bovis BCG harbouring the pGS3 vector Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
no activity in Mycobacterium bovis BCG
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+ Mycobacterium tuberculosis displays less Ald activity than the complemented Mycobacterium bovis BCG strain Mycobacterium tuberculosis pyruvate + NH3 + NADH
-
 ?

Synonyms

Synonyms Comment Organism
ALD
-
 Mycobacterium tuberculosis
L-alanine dehydrogenase
-
 Mycobacterium tuberculosis
NAD+ dependent amino acid dehydrogenase
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mycobacterium tuberculosis