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Literature summary for 1.4.1.12 extracted from

  • Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.
    A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine (2009), J. Bacteriol., 191, 3162-3167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.12

Inhibitors

Inhibitors Comment Organism Structure
2-amino-4-ketopentanoate 70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate Acetoanaerobium sticklandii
acetyl-CoA 30% inhibition in the presence of 1 mM acetyl-CoA Acetoanaerobium sticklandii
D-alanine 45% inhibition in the presence of 1 mM D-alanine Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 NADP+
-
 Acetoanaerobium sticklandii
2100
-
 NAD+
-
 Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 SDS-PAGE Acetoanaerobium sticklandii
38930
-
 calculated from cDNA Acetoanaerobium sticklandii

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-diaminopentanoate + H2O + NAD+ enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec) Acetoanaerobium sticklandii 2-amino-4-oxopentanoate + NH3 + NADH
-
 ?
2,4-diaminopentanoate + H2O + NADP+ enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec) Acetoanaerobium sticklandii 2-amino-4-oxopentanoate + NH3 + NADPH
-
 ?

Subunits

Subunits Comment Organism
homodimer gel filtration, 2 * 38000 Da Acetoanaerobium sticklandii

Synonyms

Synonyms Comment Organism
2R,4S-2,4-diaminopentanoate dehydrogenase
-
 Acetoanaerobium sticklandii
DAPDH
-
 Acetoanaerobium sticklandii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay carried out at room temperature Acetoanaerobium sticklandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 NADP+
-
 Acetoanaerobium sticklandii
34
-
 NAD+
-
 Acetoanaerobium sticklandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+ Acetoanaerobium sticklandii
NADP+ the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+ Acetoanaerobium sticklandii
pyridoxal 5'-phosphate
-
 Acetoanaerobium sticklandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 NAD+
-
 Acetoanaerobium sticklandii
2.56
-
 NADP+
-
 Acetoanaerobium sticklandii