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Literature summary for 1.4.1.13 extracted from
- Glutamate synthase from Bacillus subtilis PCI 219 (1986), J. Biochem., 99, 1087-1100.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-methionine DL-sulfoximine | - |
Bacillus subtilis |  |
| fumarate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
| L-glutamate | noncompetitive inhibitor with respect to both 2-oxoglutarate and L-glutamine | Bacillus subtilis | |
| L-malate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
| L-methionine DL-sulfoximine | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
| NAD+ | competitive inhibition with NADH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
| NADP+ | competitive inhibition with NADPH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
| p-chloromercuribenzoate | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
| pyridoxal-5'-phosphate | 52% inhibition of the glutamine-dependent reaction at 5 mM | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
NADPH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
| 0.007 | - |
NADPH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
| 0.007 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADPH, and varied concentrations of L-glutamine | Bacillus subtilis | |
| 0.01 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADH, and varied concentrations of L-glutamine | Bacillus subtilis | |
| 0.012 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADH | Bacillus subtilis | |
| 0.013 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADPH | Bacillus subtilis | |
| 0.091 | - |
NADH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
| 0.102 | - |
L-glutamine | in the presence of a fixed concentration of NADPH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
| 0.108 | - |
L-glutamine | in the presence of a fixed concentration of NADH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
| 0.113 | - |
NADH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
| 0.115 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADPH | Bacillus subtilis | |
| 0.12 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADH | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | iron-sulfur protein | Bacillus subtilis | |
| Iron | 7.4 g-atoms of non-heme iron per mol of enzyme | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
| 160000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
| 210000 | - |
sucrose density sedimentation | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ | mechanism | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH + H+ | - |
Bacillus subtilis | L-glutamate + NAD+ | - |
r | |
| L-glutamine + 2-oxoglutarate + NADPH + H+ | - |
Bacillus subtilis | L-glutamate + NADP+ | - |
? | |
| NH3 + 2-oxoglutarate + NADPH + H+ | 24% relative activity to L-glutamine with NADPH as electron donor and 6.3% relative activity to L-glutamine with NADH as electron donor | Bacillus subtilis | L-glutamate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 160000 + 1 * 56000 | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
NADH-glutamine dependent activity | Bacillus subtilis |
| 7.8 | - |
NADPH-glutamine dependent activity | Bacillus subtilis |
| 9.4 | - |
NADPH-NH3 dependent activity | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | 0.83 mol of FMN and 0.81 mol of FAD per mol of enzyme | Bacillus subtilis | |
| additional information | the enzyme contains 8.7 g atoms per mol | Bacillus subtilis | |
| NADH | 21% relative activity to NADPH | Bacillus subtilis | |
| NADPH | - |
Bacillus subtilis | |
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