Literature summary for 1.4.1.13 extracted from

Vanoni, M.A.; Fischer, F.; Ravasio, S.; Verzotti, E.; Edmondson, D.E.; Hagen, W.R.; Zanetti, G.; Curti, B.
The recombinant alpha subunit of glutamate synthase: spectroscopic and catalytic properties (1998), Biochemistry, 37, 1828-1838.

Search for more literature for 1.4.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
production of the alpha and beta subunits in Escherichia coli	Azospirillum brasilense

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Aminopyridine adenine dinucleotide phosphate	good inhibitor of the holoenzyme, competitive with respect to NADPH	Azospirillum brasilense
iodonitrotetrazolium	inhibitor of the L-glutamate:iodonitrotetrazolim oxidoreductase activity of the alpha subunit at concentrations above 0.1 mM	Azospirillum brasilense

Organism

Organism	UniProt	Comment	Textmining
Azospirillum brasilense	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34	Azospirillum brasilense

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+	-	Azospirillum brasilense	L-glutamate + NADP+	-	?
additional information	the alpha subunit catalyzes the synthesis of glutamate from L-glutamine and 2-oxoglutarate, provided that a reducing system is present, reducing system: dithionite and methyl viologen	Azospirillum brasilense	?	-	?

Cofactor

Cofactor	Comment	Organism	Structure
flavin	the alpha subunit contains FMN as flavin cofactor, 0.94 FMN bound per alpha subunit	Azospirillum brasilense
flavin	the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit	Azospirillum brasilense
additional information	the alpha subunit contains the [3Fe-4S] cluster of the enzyme	Azospirillum brasilense
NADPH	the beta subunit contains the NADPH binding site of the enzyme	Azospirillum brasilense

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Azospirillum brasilense
0.0024	-	3-Aminopyridine adenine dinucleotide phosphate	inhibitor of the holoenzyme, competitive with NADPH	Azospirillum brasilense

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Release 2023.1 (January 2023)