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Literature summary for 1.4.1.14 extracted from

  • Romero, D.; Davila, G.
    Genetic and biochemical identification of the glutamate synthase structural gene in Neurospora crassa (1986), J. Bacteriol., 167, 1043-1047.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information three en(am)-2 mutants, lacking enzyme activity, no growth on ammonia-limited conditions Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 1 * 200000, SDS-PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Neurospora crassa important role in glutamine degradation glutamate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
 strains 74-A, 73-a, fl A, fl a, am 132, en(am)-2 C24
-

Purification (Commentary)

Purification (Comment) Organism
150fold partial purification, strain am(132) Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH
-
 Neurospora crassa L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH important role in glutamine degradation Neurospora crassa glutamate + NAD+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 200000, SDS-PAGE Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Neurospora crassa