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Literature summary for 1.4.1.14 extracted from

  • Doverskog, M.; Jacobsson, U.; Chapman, B.E.; Kuchel, P.W.; Häggström, L.
    Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (Sf9) insect cells by a selective 1H/15N NMR in vitro assay (2000), J. Biotechnol., 79, 87-97.
    View publication on PubMed
Search for more literature for 1.4.1.14

Inhibitors

Inhibitors Comment Organism Structure
azaserine completely inhibited by 1 mM, glutamine analog Bombyx mori
azaserine completely inhibited by 1 mM, glutamine analog Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Spodoptera frugiperda involvement in glutamine metabolism, when glucose is in excess glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH Spodoptera frugiperda Sf9 involvement in glutamine metabolism, when glucose is in excess glutamate + NAD+
-
 ir

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-
Spodoptera frugiperda
-
 Sf9
-
Spodoptera frugiperda Sf9
-
 Sf9
-

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bombyx mori
additional information
-
-
 Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH specific for NADH Bombyx mori L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH specific for NADH Spodoptera frugiperda L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH specific for NADH Spodoptera frugiperda Sf9 L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH involvement in glutamine metabolism, when glucose is in excess Spodoptera frugiperda glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH involvement in glutamine metabolism, when glucose is in excess Spodoptera frugiperda Sf9 glutamate + NAD+
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Spodoptera frugiperda
30
-
 assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Bombyx mori

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Spodoptera frugiperda
NADH exclusively dependent on NADH as coenzyme Bombyx mori
NADH exclusively dependent on NADH as coenzyme Spodoptera frugiperda
NADH specific for NADH as reductant Bombyx mori
NADH specific for NADH as reductant Spodoptera frugiperda