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Literature summary for 1.4.1.14 extracted from

  • Chichkova, S.; Arellano, J.; Vance, C.P.; Hernandez, G.
    Transgenic tobacco plants that overexpress alfalfa NADH-glutamate synthase have higher carbon and nitrogen content (2001), J. Exp. Bot., 52, 2079-2087.
    View publication on PubMed
Search for more literature for 1.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Oryza sativa
isolation and characterization of NADH-GOGAT gene with 22 exons and 21 introns, isolation of 7.2 kbp cDNA encoding the complete enzyme Medicago sativa
overexpression of chimeric NADH-GOGAT gene from Medicago sativa fused to the CaMV 35S promoter in transgenic Nicotiana tabacum Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Oryza sativa
-
 glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH Medicago sativa plays a critical role in the assimilation of symbiotically fixed nitrogen in nodules and during pollen development glutamate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
-
-
Nicotiana tabacum
-
 cv. Xanthi, transgenic tobacco that overexpress NADH-GOGAT from Medicago sativa, higher capacity to assimilate nitrogen due to a higher NADH-GOGAT activity
-
Oryza sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification
-
 Oryza sativa
proteolytic modification primary translation product is a 240 kDa protein, that contains a N-terminal 101 amino acid presequence resulting in a processed protein of 229 kDa Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
flower enzyme activity is detectable in flowers Medicago sativa
-
leaf
-
 Oryza sativa
-
leaf low concentrations Nicotiana tabacum
-
root
-
 Oryza sativa
-
root low concentrations, but 15-40% higher enzyme activity in the roots of transgenic plants than of control plants Nicotiana tabacum
-
root nodule enzyme activity increases markedly during development of effective root nodules Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme specific activity of transformants is significantly greater than that of control plants Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH
-
 Nicotiana tabacum L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH
-
 Medicago sativa L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH
-
 Oryza sativa L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH
-
 Oryza sativa glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH plays a critical role in the assimilation of symbiotically fixed nitrogen in nodules and during pollen development Medicago sativa glutamate + NAD+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Nicotiana tabacum
NADH
-
 Medicago sativa
NADH
-
 Oryza sativa