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Literature summary for 1.4.1.14 extracted from
- Functional characterization of key enzymes involved in L-glutamate synthesis and degradation in the thermotolerant and methylotrophic bacterium Bacillus methanolicus (2013), Appl. Environ. Microbiol., 79, 5321-5328.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GltB | GltA and not GltA2 is dependent on GltB for GOGAT activity in vivo | Bacillus methanolicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes gltA and gltA2, phylogenetic analysis | Bacillus methanolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
L-glutamate | pH 8.5, 45°C, GltA and GltA2 | Bacillus methanolicus |  |
| 0.0014 | - |
2-oxoglutarate | pH 8.5, 45°C, GltA and GltA2 | Bacillus methanolicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bacillus methanolicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-glutamate + NAD+ | Bacillus methanolicus | - |
L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r | |
| 2 L-glutamate + NAD+ | Bacillus methanolicus ATCC 53907 | - |
L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus methanolicus | - |
two isozymes encoded by genes gltA and gltA2 of the gltAB operon | - |
| Bacillus methanolicus ATCC 53907 | - |
two isozymes encoded by genes gltA and gltA2 of the gltAB operon | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-glutamate + NAD+ | - |
Bacillus methanolicus | L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r | |
| 2 L-glutamate + NAD+ | - |
Bacillus methanolicus ATCC 53907 | L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gltA | - |
Bacillus methanolicus |
| GltA2 | - |
Bacillus methanolicus |
| GOGAT | - |
Bacillus methanolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Bacillus methanolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Bacillus methanolicus |
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