Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli | Achromobacter denitrificans |
Crystallization (Comment) | Organism |
---|---|
using the hanging drop method | Achromobacter denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-amino-n-caproate | inhibition 20% by 10 mM 6-amino-n-caproate | Achromobacter denitrificans | |
HgCl2 | - |
Achromobacter denitrificans | |
L-leucine | inhibition 50% by 10 mM L-leucine | Achromobacter denitrificans | |
L-lysine | - |
Achromobacter denitrificans | |
L-norleucine | inhibition 20% by 10 mM L-norleucine | Achromobacter denitrificans | |
L-ornithine | inhibition 10% by 10 mM L-ornithine | Achromobacter denitrificans | |
N-ethylmaleimide | - |
Achromobacter denitrificans | |
p-chloromercuribenzoate | - |
Achromobacter denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NAD+ | - |
Achromobacter denitrificans | |
0.11 | - |
deamino-NAD+ | - |
Achromobacter denitrificans | |
0.37 | - |
nicotinamide guanine dinucleotide | - |
Achromobacter denitrificans | |
1.32 | - |
3-acetylpyridine-NAD+ | - |
Achromobacter denitrificans | |
5 | - |
L-lysine | - |
Achromobacter denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39360 | - |
predicted from cDNA | Achromobacter denitrificans |
40000 | - |
SDS-PAGE | Achromobacter denitrificans |
80000 | - |
gel filtration, homodimer | Achromobacter denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter denitrificans | Q3S559 | - |
- |
Purification (Comment) | Organism |
---|---|
enzyme is purified approximately 100fold to homogeneity with a 15% yield | Achromobacter denitrificans |
Storage Stability | Organism |
---|---|
4°C, full activity is maintained for 2 months | Achromobacter denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 3-acetylpyridine-NAD+ + H2O | - |
Achromobacter denitrificans | alpha-aminoadipate delta-semialdehyde + NH3 + 3-acetylpyridine-NADH | - |
? | |
L-lysine + deamino-NAD+ + H2O | - |
Achromobacter denitrificans | alpha-aminoadipate delta-semialdehyde + NH3 + deamino-NADH | - |
? | |
L-lysine + NAD+ + H2O | - |
Achromobacter denitrificans | alpha-aminoadipate delta-semialdehyde + NH3 + NADH | - |
? | |
L-lysine + nicotinamide guanine dinucleotide + H2O | - |
Achromobacter denitrificans | alpha-aminoadipate delta-semialdehyde + NH3 + nicotinamide guanine dinucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40000 Da, gel filtration, enzyme elutes as a hexamer when a high concentration of L-lysine (10 mM) is supplemented to both the enzyme and the elution buffer | Achromobacter denitrificans |
homodimer | 2 * 40000 Da, gel filtration | Achromobacter denitrificans |
Synonyms | Comment | Organism |
---|---|---|
Lys 6-DH | - |
Achromobacter denitrificans |
lysine 6-dehydrogenase | - |
Achromobacter denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Achromobacter denitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
at 30°C enzyme activity is kept for 3 days and 70% of the activity remains after 7 days | Achromobacter denitrificans |
50 | - |
enzyme is stable up to 50°C when heated for 10 min in KP buffer (pH 7.4) | Achromobacter denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.7 | - |
optimal pH | Achromobacter denitrificans |
10 | - |
assay at | Achromobacter denitrificans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Achromobacter denitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine-NAD+ | - |
Achromobacter denitrificans | |
deamino-NAD+ | - |
Achromobacter denitrificans | |
NAD+ | no activity with NADP+ | Achromobacter denitrificans | |
nicotinamide guanine dinucleotide | - |
Achromobacter denitrificans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
L-lysine | - |
Achromobacter denitrificans |