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Literature summary for 1.4.1.18 extracted from

  • Ruldeekulthamrong, P.; Maeda, S.; Kato, S.; Shinji, N.; Sittipraneed, S.; Packdibamrung, K.; Misono, H.
    Molecular characterization of lysine 6-dehydrogenase from Achromobacter denitrificans (2008), BMB Rep., 41, 790-795.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli Achromobacter denitrificans

Crystallization (Commentary)

Crystallization (Comment) Organism
using the hanging drop method Achromobacter denitrificans

Inhibitors

Inhibitors Comment Organism Structure
6-amino-n-caproate inhibition 20% by 10 mM 6-amino-n-caproate Achromobacter denitrificans
HgCl2
-
Achromobacter denitrificans
L-leucine inhibition 50% by 10 mM L-leucine Achromobacter denitrificans
L-lysine
-
Achromobacter denitrificans
L-norleucine inhibition 20% by 10 mM L-norleucine Achromobacter denitrificans
L-ornithine inhibition 10% by 10 mM L-ornithine Achromobacter denitrificans
N-ethylmaleimide
-
Achromobacter denitrificans
p-chloromercuribenzoate
-
Achromobacter denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
NAD+
-
Achromobacter denitrificans
0.11
-
deamino-NAD+
-
Achromobacter denitrificans
0.37
-
nicotinamide guanine dinucleotide
-
Achromobacter denitrificans
1.32
-
3-acetylpyridine-NAD+
-
Achromobacter denitrificans
5
-
L-lysine
-
Achromobacter denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39360
-
predicted from cDNA Achromobacter denitrificans
40000
-
SDS-PAGE Achromobacter denitrificans
80000
-
gel filtration, homodimer Achromobacter denitrificans

Organism

Organism UniProt Comment Textmining
Achromobacter denitrificans Q3S559
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme is purified approximately 100fold to homogeneity with a 15% yield Achromobacter denitrificans

Storage Stability

Storage Stability Organism
4°C, full activity is maintained for 2 months Achromobacter denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 3-acetylpyridine-NAD+ + H2O
-
Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + 3-acetylpyridine-NADH
-
?
L-lysine + deamino-NAD+ + H2O
-
Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + deamino-NADH
-
?
L-lysine + NAD+ + H2O
-
Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + NADH
-
?
L-lysine + nicotinamide guanine dinucleotide + H2O
-
Achromobacter denitrificans alpha-aminoadipate delta-semialdehyde + NH3 + nicotinamide guanine dinucleotide
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 40000 Da, gel filtration, enzyme elutes as a hexamer when a high concentration of L-lysine (10 mM) is supplemented to both the enzyme and the elution buffer Achromobacter denitrificans
homodimer 2 * 40000 Da, gel filtration Achromobacter denitrificans

Synonyms

Synonyms Comment Organism
Lys 6-DH
-
Achromobacter denitrificans
lysine 6-dehydrogenase
-
Achromobacter denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Achromobacter denitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
at 30°C enzyme activity is kept for 3 days and 70% of the activity remains after 7 days Achromobacter denitrificans
50
-
enzyme is stable up to 50°C when heated for 10 min in KP buffer (pH 7.4) Achromobacter denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
optimal pH Achromobacter denitrificans
10
-
assay at Achromobacter denitrificans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7
-
Achromobacter denitrificans

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine-NAD+
-
Achromobacter denitrificans
deamino-NAD+
-
Achromobacter denitrificans
NAD+ no activity with NADP+ Achromobacter denitrificans
nicotinamide guanine dinucleotide
-
Achromobacter denitrificans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2
-
L-lysine
-
Achromobacter denitrificans