Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.2 extracted from

  • Meredith, M.J.; Gronostajski, R.M.; Schmidt, R.R.
    Physical and kinetic properties of the nicotinamide adenine dinucleotide-specific glutamate dehydrogenase purified from Chlorella sorokiniana (1978), Plant Physiol., 61, 967-974.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.2

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Chlorella sorokiniana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Chlorella sorokiniana
180000
-
 gel filtration Chlorella sorokiniana

Organism

Organism UniProt Comment Textmining
Chlorella sorokiniana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlorella sorokiniana

Reaction

Reaction Comment Organism Reaction ID
L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH + H+ bi uni uni ping-pong addition sequence Chlorella sorokiniana
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
-
 Chlorella sorokiniana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Chlorella sorokiniana 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Chlorella sorokiniana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 reductive amination Chlorella sorokiniana
9
-
 oxidative deamination Chlorella sorokiniana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Chlorella sorokiniana
NADH
-
 Chlorella sorokiniana