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Literature summary for 1.4.1.2 extracted from

  • Consalvi, V.; Chiaraluce, R.; Millevoi, S.; Pasquo, A.; Politi, L.; De Rosa, M.; Scandurra, R.
    NAD-dependent glutamate dehydrogenase from the thermophilic eubacterium Bacillus acidocaldarius (1994), Comp. Biochem. Physiol. B, 109, 691-699.
No PubMed abstract available
Search for more literature for 1.4.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADH
-
 Alicyclobacillus acidocaldarius
0.35
-
 NAD+
-
 Alicyclobacillus acidocaldarius
25
-
 L-glutamate
-
 Alicyclobacillus acidocaldarius
60
-
 NH4+
-
 Alicyclobacillus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 6 * 48000, SDS-PAGE Alicyclobacillus acidocaldarius
290000
-
 gel filtration Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
 Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
 Alicyclobacillus acidocaldarius L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH4+ + NADPH
-
 Alicyclobacillus acidocaldarius L-glutamate + NADP+
-
 ?
L-glutamate + H2O + NAD+
-
 Alicyclobacillus acidocaldarius 2-oxoglutarate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 48000, SDS-PAGE Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 forward reaction Alicyclobacillus acidocaldarius
9
-
 reverse reaction Alicyclobacillus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Alicyclobacillus acidocaldarius