Literature summary for 1.4.1.2 extracted from

Nguyen, K.T.; Nguyen, L.T.; Kopecky, J.; Behal, V.
Properties of NAD-dependent glutamate dehydrogenase from the tylosin producer Streptomyces fradiae (1997), Can. J. Microbiol., 43, 1005-1010.

No PubMed abstract available

Search for more literature for 1.4.1.2

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Streptomyces fradiae
iodoacetamide	-	Streptomyces fradiae
Mn2+	weak	Streptomyces fradiae
Ni2+	-	Streptomyces fradiae
p-hydroxymercuribenzoate	-	Streptomyces fradiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	NADH	-	Streptomyces fradiae
0.3	-	NAD+	-	Streptomyces fradiae
3.4	-	2-oxoglutarate	-	Streptomyces fradiae
14.2	-	NH4+	-	Streptomyces fradiae
32.2	-	L-glutamate	-	Streptomyces fradiae

Organism

Organism	UniProt	Comment	Textmining
Streptomyces fradiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Streptomyces fradiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + NH4+ + NADPH	-	Streptomyces fradiae	L-glutamate + NADP+	-	?
L-glutamate + H2O + NAD+	-	Streptomyces fradiae	2-oxoglutarate + NH3 + NADH + H+	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	35	-	Streptomyces fradiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	-	reductive amination	Streptomyces fradiae
9.2	-	oxidative deamination	Streptomyces fradiae

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Streptomyces fradiae

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Release 2023.1 (January 2023)