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Literature summary for 1.4.1.2 extracted from

  • Kawakami, R.; Sakuraba, H.; Ohshima, T.
    Gene cloning and characterization of the very large NAD-dependent L-glutamate dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil (2007), J. Bacteriol., 189, 5626-5633.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
D-arginine 212% activity in the presence of 10 mM D-arginine Janthinobacterium lividum
L-arginine 936% activity in the presence of 10 mM L-arginine Janthinobacterium lividum
L-asparagine 118% activity in the presence of 10 mM L-asparagine Janthinobacterium lividum
L-aspartate 1735% activity in the presence of 10 mM L-aspartate Janthinobacterium lividum
L-histidine 155% activity in the presence of 10 mM L-histidine Janthinobacterium lividum
L-lysine 161% activity in the presence of 10 mM L-lysine Janthinobacterium lividum
L-methionine 206% activity in the presence of 10 mM L-methionine Janthinobacterium lividum
L-tryptophan 235% activity in the presence of 10 mM L-tryptophan Janthinobacterium lividum
Zn2+ 224% activity in the presence of 1 mM Zn2+ Janthinobacterium lividum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Janthinobacterium lividum

Inhibitors

Inhibitors Comment Organism Structure
citrate 67% activity in the presence of 10 mM citrate Janthinobacterium lividum
Co2+ 82% activity in the presence of 1 mM Co2+ Janthinobacterium lividum
D-asparagine 93% activity in the presence of 10 mM D-asparagine Janthinobacterium lividum
D-Aspartate 90% activity in the presence of 10 mM D-aspartate Janthinobacterium lividum
D-glutamate 94% activity in the presence of 10 mM D-glutamate Janthinobacterium lividum
D-glutamine 95% activity in the presence of 10 mM D-glutamine Janthinobacterium lividum
fumarate 32% activity in the presence of 10 mM fumarate Janthinobacterium lividum
Hg2+ no activity in the presence of 1 mM Hg2+ Janthinobacterium lividum
isocitrate 49% activity in the presence of 10 mM isocitrate Janthinobacterium lividum
L-glutamate substrate inhibition at L-glutamate concentrations above 20 mM Janthinobacterium lividum
L-glutamine 89% activity in the presence of 10 mM L-glutamine Janthinobacterium lividum
L-ornithine 83% activity in the presence of 10 mM L-ornithine Janthinobacterium lividum
malate 65% activity in the presence of 10 mM malate Janthinobacterium lividum
Mg2+ 64% activity in the presence of 1 mM Mg2+ Janthinobacterium lividum
Mn2+ 52% activity in the presence of 1 mM Mn2+ Janthinobacterium lividum
additional information not inhibited by NAD+ Janthinobacterium lividum
Ni2+ 77% activity in the presence of 1 mM Ni2+ Janthinobacterium lividum
succinate 36% activity in the presence of 10 mM succinate Janthinobacterium lividum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 NAD+ in the presence of 20 mM L-glutamate, in 100 mM glycine/NaOH (pH 9.5), at 25°C Janthinobacterium lividum
7.1
-
 L-glutamate in the presence of 5 mM NAD+, in 100 mM glycine/NaOH (pH 9.5), at 25°C Janthinobacterium lividum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
169360
-
 6 * 169360, deduced from amino acid sequence Janthinobacterium lividum
170000
-
 6 * 170000, SDS-PAGE Janthinobacterium lividum

Organism

Organism UniProt Comment Textmining
Janthinobacterium lividum A5LH94 fragment
-
Janthinobacterium lividum UTB1302 A5LH94 fragment
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and Superdex 200 gel filtration Janthinobacterium lividum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
 recombinant enzyme from crude cell extract, at 25°C Janthinobacterium lividum
3.1
-
 purified recombinant enzyme, at 25°C Janthinobacterium lividum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Janthinobacterium lividum 2-oxoglutarate + NH3 + NADH
-
 ?
L-glutamate + H2O + NAD+
-
 Janthinobacterium lividum UTB1302 2-oxoglutarate + NH3 + NADH
-
 ?
additional information inert reaction with L-glutamine, L-aspartate, L-alanine, L-leucine, L-valine, L-lysine, L-2-aminobutyrate, L-methionine, L-ornithine, L-phenylalanine, L-arginine, L-tryptophan, L-methionine, L-histidine, D-glutamate, and D-aspartate, the enzyme is not active with NADP+ Janthinobacterium lividum ?
-
 ?
additional information inert reaction with L-glutamine, L-aspartate, L-alanine, L-leucine, L-valine, L-lysine, L-2-aminobutyrate, L-methionine, L-ornithine, L-phenylalanine, L-arginine, L-tryptophan, L-methionine, L-histidine, D-glutamate, and D-aspartate, the enzyme is not active with NADP+ Janthinobacterium lividum UTB1302 ?
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 169360, deduced from amino acid sequence Janthinobacterium lividum
homohexamer 6 * 170000, SDS-PAGE Janthinobacterium lividum

Synonyms

Synonyms Comment Organism
NAD-dependent L-glutamate dehydrogenase
-
 Janthinobacterium lividum
NAD-GDH
-
 Janthinobacterium lividum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 in glycine-NaOH buffer at pH 9.0 Janthinobacterium lividum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme is stable for 10 min at temperatures up to 40°C but is completely inactivated by incubation for 10 min at 50°C Janthinobacterium lividum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 in glycine-NaOH buffer at 40°C Janthinobacterium lividum

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent Janthinobacterium lividum