Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is activated by heat above 70°C, urea and organic solvents, but not by salts. Activation of recombinant enzyme by urea and heat, that is purified as inactive enzyme at 4°C | Pyrobaculum islandicum | |
Urea | activates the enzyme irreversibly at 5 M | Pyrobaculum islandicum |
Cloned (Comment) | Organism |
---|---|
expression of inactive enzyme in Escherichia coli | Pyrobaculum islandicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
280000 | - |
- |
Pyrobaculum islandicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Pyrobaculum islandicum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inactivate recombinant enzyme expressed from Escherichia coli by affinity and anion exchange chromatography, followed by gel filtrration, activation by 5 M urea and 70°c | Pyrobaculum islandicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxopentanoate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 47000, SDS-PAGE | Pyrobaculum islandicum |
More | three-dimensional structure and structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent glutamate dehydrogenase | - |
Pyrobaculum islandicum |
NAD-GDH | - |
Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
at pH 9.7 | Pyrobaculum islandicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme shows extremely high temperature stability, molecular mechanism and comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |
100 | - |
pH 7.2, 120 min, stable | Pyrobaculum islandicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
amination | Pyrobaculum islandicum |
9.7 | - |
deamination | Pyrobaculum islandicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrobaculum islandicum | |
NADH | - |
Pyrobaculum islandicum |
General Information | Comment | Organism |
---|---|---|
additional information | structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview | Pyrobaculum islandicum |