Protein Variants | Comment | Organism |
---|---|---|
N145A | 4-trifluoromethyl-phenylpyruvate is the preferred substrate, the reaction velocity at elevated substrate concentration (5 mM) is almost 10 times higher compared to the wild type enzyme | Lysinibacillus sphaericus |
N145L | 4-methoxy-phenylpyruvate is the preferred substrate | Lysinibacillus sphaericus |
N145V | 4-fluoro-phenylpyruvate is the preferred substrate | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
4-trifluoromethyl-phenylpyruvate | mutant enzyme N145A, at 25°C inTris buffer 50mM pH 8.5, with NADH (0.1 mM), KCl (100 mM) and NH4Cl (400 mM) | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-fluoro-phenylpyruvate + NH3 + NADH | preferred substrate of mutant N145V | Lysinibacillus sphaericus | 4-fluoro-L-phenylalanine + H2O + NAD+ | - |
r | |
4-methoxy-phenylpyruvate + NH3 + NADH | preferred substrate of mutant N145L | Lysinibacillus sphaericus | 4-methoxy-L-phenylalanine + H2O + NAD+ | - |
r | |
4-trifluoromethyl-phenylpyruvate + NH3 + NADH | preferred substrate of mutant N145A | Lysinibacillus sphaericus | 4-trifluoromethyl-L-phenylalanine + H2O + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus |