Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Bacillus badius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Bacillus badius | |
0.2 | - |
L-Phe | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Bacillus badius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
in aqueous two-phase systems composed of polyethylene glycol 6000 and ammonium sulfate with 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and 13% (w/w) NaCl at pH 8.0 | Bacillus badius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the 474fold purified enzyme exhibits a specific activity of 10424.97 units/mg | Bacillus badius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-phenylalanine:NAD+ oxidoreductase, deaminating | - |
Bacillus badius |
PheDH | - |
Bacillus badius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus badius |