Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli via plasmids | Lysinibacillus sphaericus |
Protein Variants | Comment | Organism |
---|---|---|
D126N | mutant without enzymatic activity | Lysinibacillus sphaericus |
D209G | mutant named 1stA7, active with DL-propargylglycine | Lysinibacillus sphaericus |
D209G/Q18H/I336F | mutant named 1stA7/1stB6, active with DL-propargylglycine | Lysinibacillus sphaericus |
E313G | mutant named H7H10, active with DL-propargylglycine | Lysinibacillus sphaericus |
F110Y/G124C/G293A | mutant named H14A12, active with DL-propargylglycine | Lysinibacillus sphaericus |
G124A | mutant named 9F5, active with DL-propargylglycine | Lysinibacillus sphaericus |
G124A/E313G | mutant named 25B12, active with DL-propargylglycine | Lysinibacillus sphaericus |
G124A/E313N | mutant named 20E1, active with DL-propargylglycine | Lysinibacillus sphaericus |
K224Q/L283F | mutant named 2ndB10, active with DL-propargylglycine | Lysinibacillus sphaericus |
K224Q/L283F/E313G | mutant named 2ndB10/H7H10, active with DL-propargylglycine | Lysinibacillus sphaericus |
Q18H/I336F | mutant named 1stB6, active with DL-propargylglycine | Lysinibacillus sphaericus |
Q18H/I336F/E313G | mutant named 1stB6/H7H10, active with DL-propargylglycine | Lysinibacillus sphaericus |
V135I/I308N/Q363H | mutant named H21D1, active with DL-propargylglycine | Lysinibacillus sphaericus |
V33A/A206D/L283F | mutant named 2ndC2., active with DL-propargylglycine | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
L-phenylalanine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
0.02 | - |
L-phenylalanine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
0.035 | - |
L-phenylalanine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
0.04 | - |
L-phenylalanine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
0.06 | - |
L-phenylalanine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
0.07 | - |
L-phenylalanine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
0.07 | - |
L-phenylalanine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
0.12 | - |
L-phenylalanine | mutation E313G | Lysinibacillus sphaericus | |
0.67 | - |
L-phenylalanine | mutation D209G | Lysinibacillus sphaericus | |
1.4 | - |
L-phenylalanine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus | |
1.51 | - |
L-phenylalanine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
2 | 3 | DL-propargylglycine | mutations G124A/E313G | Lysinibacillus sphaericus | |
4.5 | - |
L-phenylalanine | mutations G124A/E313N | Lysinibacillus sphaericus | |
7.9 | - |
L-phenylalanine | mutation G124A | Lysinibacillus sphaericus | |
9.6 | - |
L-phenylalanine | mutations G124A/E313G | Lysinibacillus sphaericus | |
28 | - |
DL-propargylglycine | mutation E313G | Lysinibacillus sphaericus | |
28 | - |
DL-propargylglycine | mutation G124A | Lysinibacillus sphaericus | |
32 | - |
DL-propargylglycine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
32 | - |
DL-propargylglycine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
34 | - |
DL-propargylglycine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
39 | - |
DL-propargylglycine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
42 | - |
DL-propargylglycine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
44 | - |
DL-propargylglycine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
48 | - |
DL-propargylglycine | mutations G124A/E313N | Lysinibacillus sphaericus | |
58 | - |
DL-propargylglycine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
84 | - |
DL-propargylglycine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
134 | - |
DL-propargylglycine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus | |
298 | - |
DL-propargylglycine | mutation D209G | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-propargylglycine + phenazine ethosulfate + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloridehydrate + NAD+ | substrate is poorly fertilized by wild type enzyme, but using muteins with different mutations higher turnover can be achieved | Lysinibacillus sphaericus | ? + NADH | - |
? | |
L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | phenylpyruvate + NH3 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
phenylalanine dehydrogenase | - |
Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
DL-propargylglycine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
0.57 | - |
DL-propargylglycine | mutations G124A/E313N | Lysinibacillus sphaericus | |
0.59 | - |
DL-propargylglycine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
0.608 | - |
L-phenylalanine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
0.69 | - |
DL-propargylglycine | mutation G124A | Lysinibacillus sphaericus | |
0.722 | - |
DL-propargylglycine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus | |
0.759 | - |
DL-propargylglycine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
0.91 | - |
L-phenylalanine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
0.92 | - |
DL-propargylglycine | mutations G124A/E313G | Lysinibacillus sphaericus | |
1.02 | - |
DL-propargylglycine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
1.07 | - |
DL-propargylglycine | mutation E313G | Lysinibacillus sphaericus | |
1.09 | - |
L-phenylalanine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
1.12 | - |
L-phenylalanine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
1.15 | - |
DL-propargylglycine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
1.3 | - |
DL-propargylglycine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
1.42 | - |
DL-propargylglycine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
1.49 | - |
DL-propargylglycine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
1.82 | - |
DL-propargylglycine | mutation D209G | Lysinibacillus sphaericus | |
2.6 | - |
L-phenylalanine | mutation G124A | Lysinibacillus sphaericus | |
3.07 | - |
L-phenylalanine | mutations G124A/E313N | Lysinibacillus sphaericus | |
3.16 | - |
L-phenylalanine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
4.08 | - |
L-phenylalanine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
4.66 | - |
L-phenylalanine | mutations G124A/E313G | Lysinibacillus sphaericus | |
5.8 | - |
L-phenylalanine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
6.56 | - |
L-phenylalanine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
32.1 | - |
L-phenylalanine | mutation E313G | Lysinibacillus sphaericus | |
56.1 | - |
L-phenylalanine | mutation D209G | Lysinibacillus sphaericus | |
57.1 | - |
L-phenylalanine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
DL-propargylglycine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus | |
0.0061 | - |
DL-propargylglycine | mutation D209G | Lysinibacillus sphaericus | |
0.012 | - |
DL-propargylglycine | mutations G124A/E313N | Lysinibacillus sphaericus | |
0.015 | - |
DL-propargylglycine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
0.015 | - |
DL-propargylglycine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
0.0176 | - |
DL-propargylglycine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
0.0183 | - |
DL-propargylglycine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
0.022 | - |
DL-propargylglycine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
0.025 | - |
DL-propargylglycine | mutation G124A | Lysinibacillus sphaericus | |
0.032 | - |
DL-propargylglycine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
0.032 | - |
DL-propargylglycine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
0.034 | - |
DL-propargylglycine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
0.0384 | - |
DL-propargylglycine | mutation E313G | Lysinibacillus sphaericus | |
0.04 | - |
DL-propargylglycine | mutations G124A/E313G | Lysinibacillus sphaericus | |
0.33 | - |
L-phenylalanine | mutation G124A | Lysinibacillus sphaericus | |
0.49 | - |
L-phenylalanine | mutations G124A/E313G | Lysinibacillus sphaericus | |
0.68 | - |
L-phenylalanine | mutations G124A/E313N | Lysinibacillus sphaericus | |
0.74 | - |
L-phenylalanine | mutations F110Y/G124C/G293A | Lysinibacillus sphaericus | |
17.4 | - |
L-phenylalanine | mutations V135I/I308N/Q363H | Lysinibacillus sphaericus | |
40.5 | - |
L-phenylalanine | wild type enzyme of Bacillus sphaericus | Lysinibacillus sphaericus | |
50.6 | - |
L-phenylalanine | mutations Q18H/I336F/E313G | Lysinibacillus sphaericus | |
55.6 | - |
L-phenylalanine | mutations K224Q/L283F/E313G | Lysinibacillus sphaericus | |
58.3 | - |
L-phenylalanine | mutations D209G/Q18H/I336F | Lysinibacillus sphaericus | |
68.4 | - |
L-phenylalanine | mutations K224Q/L283F | Lysinibacillus sphaericus | |
84.9 | - |
L-phenylalanine | mutation D209G | Lysinibacillus sphaericus | |
91.6 | - |
L-phenylalanine | mutations Q18H/I336F | Lysinibacillus sphaericus | |
102.5 | - |
L-phenylalanine | mutations V33A/A206D/L283F | Lysinibacillus sphaericus | |
279 | - |
L-phenylalanine | mutation E313G | Lysinibacillus sphaericus |