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Literature summary for 1.4.1.21 extracted from

  • Yang, Z.; Savchenko, A.; Yakunin, A.; Zhang, R.; Edwards, A.; Arrowsmith, C.; Tong, L.
    Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of TM1643 (2003), J. Biol. Chem., 278, 8804-8808.
    View publication on PubMed
Search for more literature for 1.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermotoga maritima

General Stability

General Stability Organism
stable Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
L-malate competitive Thermotoga maritima
NH4+ competitive Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 L-Asp cofactor NAD+ Thermotoga maritima
0.067
-
 L-aspartate +/- 0.008, with NAD+ Thermotoga maritima
0.25
-
 NAD+
-
 Thermotoga maritima
0.25
-
 NAD+ +/- 0.02 Thermotoga maritima
0.72
-
 NADP+
-
 Thermotoga maritima
0.72
-
 NADP+ +/- 0.04 Thermotoga maritima
1.2
-
 L-Asp cofactor: NADP+ Thermotoga maritima
1.2
-
 L-aspartate +/- 0.05, with NADP+ Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + NAD(P)+ Thermotoga maritima
-
 oxaloacetate + NH4+ + NAD(P)H
-
 ?
oxaloacetate + NAD(P)H + NH4+ Thermotoga maritima
-
 L-aspartate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1X6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.63
-
 +/- 0.15, L-aspartate with NAD+ Thermotoga maritima
3.36
-
 +/- 0.25, NAD+ with L-aspartate Thermotoga maritima
9.51
-
 +/- 0.17, L-aspartate with NADP+ Thermotoga maritima
12.32
-
 +/- 0.88, NADP+ with L-aspartate Thermotoga maritima

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + H2O + NAD+
-
 Thermotoga maritima oxaloacetate + NH3 + NADH + H+
-
 r
L-aspartate + NAD(P)+
-
 Thermotoga maritima oxaloacetate + NH4+ + NAD(P)H
-
 ?
L-aspartate + NAD(P)+
-
 Thermotoga maritima oxaloacetate + NH4+ + NAD(P)H
-
 r
oxaloacetate + NAD(P)H + NH4+
-
 Thermotoga maritima L-aspartate + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
dimer crystal structure Thermotoga maritima
dimer from crystal structure Thermotoga maritima

Synonyms

Synonyms Comment Organism
L-aspartate dehydrogenase
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
 L-Asp cofactor NAD+ Thermotoga maritima
0.78
-
 L-aspartate +/- 0.02, with NAD+ Thermotoga maritima
1.2
-
 NAD+
-
 Thermotoga maritima
1.2
-
 NAD+ +/- 0.04, with L-aspartate Thermotoga maritima
4.9
-
 L-Asp cofactor NADP+ Thermotoga maritima
4.9
-
 L-aspartate +/- 0.09, with NADP+ Thermotoga maritima
7.2
-
 NADP+
-
 Thermotoga maritima
7.2
-
 NADP+ +/- 0.17, with L-aspartate Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 oxaloacetate animation Thermotoga maritima
8
-
 oxaloacetate amination Thermotoga maritima
9.8
-
 L-Asp oxidation Thermotoga maritima
9.8
-
 L-aspartate oxidation Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 L-Asp oxidation Thermotoga maritima
8 10.5 L-aspartate oxidation Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermotoga maritima
NADH
-
 Thermotoga maritima
NADP+
-
 Thermotoga maritima
NADPH
-
 Thermotoga maritima

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.02
-
 L-malate +/- 0.48 Thermotoga maritima
32.5
-
 NH4+ +/- 4.9 Thermotoga maritima