Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-Dithiopyridine | 0.01 mM, 95% inhibition | Alcaligenes faecalis | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.01 mM, 95% inhibition | Alcaligenes faecalis | |
CuSO4 | 1 mM, 13% inhibition | Alcaligenes faecalis | |
HgCl2 | 0.1 mM, 91% inhibition | Alcaligenes faecalis | |
additional information | not inhibitory at 1 mM: ATP, ADP, AMP, GTP, GDP, MgSO4, MgCl2, ZnCl2, COSO4, BaCl2, EDTA and o-phenanthroline | Alcaligenes faecalis | |
N-ethylmaleimide | 0.01 mM, 73% inhibition | Alcaligenes faecalis | |
p-chloromercuribenzoate | 0.001 mM, 95% inhibition | Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Alcaligenes faecalis |
72000 | - |
gel filtration | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 22% of the activity with 2-oxoisovalerate | Alcaligenes faecalis | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxohexanoate + NH3 + NADH + H+ | 6.3% of the activity with 2-oxoisovalerate | Alcaligenes faecalis | L-norleucine + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | 58% of the activity with 2-oxoisovalerate | Alcaligenes faecalis | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Alcaligenes faecalis | L-valine + H2O + NAD+ | - |
r | |
2-oxovalerate + H2O + NAD+ | 5.0% of the activity with 2-oxoisovalerate | Alcaligenes faecalis | L-norvaline + NH3 + NADH + H+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 68% of the activity with 2-oxoisovalerate | Alcaligenes faecalis | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 33% of the activity with L-valine | Alcaligenes faecalis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 72% of the activity with L-valine | Alcaligenes faecalis | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 73% of the activity with L-valine | Alcaligenes faecalis | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | 16% of the activity with L-valine | Alcaligenes faecalis | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 44% of the activity with L-valine | Alcaligenes faecalis | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Alcaligenes faecalis | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Alcaligenes faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Alcaligenes faecalis |
45 | - |
5 min, 50% loss of activity | Alcaligenes faecalis |
50 | - |
5 min, complete loss of activity | Alcaligenes faecalis |
55 | - |
5 min, presence of 20% glycerol, retains full activity | Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination | Alcaligenes faecalis |
10.7 | - |
oxidative demaination | Alcaligenes faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.7 | stable for at least 5 min | Alcaligenes faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine-deamino-NAD+ | 150% of the activity with NAD+ | Alcaligenes faecalis | |
3-acetylpyridine-NAD+ | 714% of the activity with NAD+ | Alcaligenes faecalis | |
3-pyridinealdehyde-NAD+ | 173% of the activity with NAD+ | Alcaligenes faecalis | |
deamino-NAD+ | 440% of the activity with NAD+ | Alcaligenes faecalis | |
additional information | no cofactor: NADP+ | Alcaligenes faecalis | |
NAD+ | - |
Alcaligenes faecalis | |
NADH | - |
Alcaligenes faecalis |