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Literature summary for 1.4.1.3 extracted from
- Purification and some properties of glutamate dehydrogenase from the mealworm fat body (1988), Insect Biochem., 18, 101-106.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | stimulation | Tenebrio molitor |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
6 * 57000, SDS-PAGE | Tenebrio molitor |
| 340000 | - |
gel filtration | Tenebrio molitor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tenebrio molitor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tenebrio molitor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fat body | - |
Tenebrio molitor | - |
| larva | - |
Tenebrio molitor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 126.4 | - |
cofactor NADH | Tenebrio molitor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD(P)+ | - |
Tenebrio molitor | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 57000, SDS-PAGE | Tenebrio molitor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
NAD+ + L-glutamate | Tenebrio molitor |
| 35 | - |
NADH + NH3 + 2-oxoglutarate | Tenebrio molitor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 714 | - |
glutamate | cofactor NADH, + 1 mM ADP | Tenebrio molitor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.75 | - |
NADPH + NH3 + 2-oxoglutarate | Tenebrio molitor |
| 7.5 | - |
NADP+ + L-glutamate, NADH + NH3 + 2-oxoglutarate | Tenebrio molitor |
| 8 | - |
NAD+ + L-glutamate | Tenebrio molitor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | preferred cofactor | Tenebrio molitor | |
| NADPH | - |
Tenebrio molitor | |
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