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Literature summary for 1.4.1.3 extracted from
- Purification and characterization of glutamate dehydrogenase as another isoprotein binding to the membrane of rough endoplasmic reticulum (1999), J. Cell. Biochem., 76, 244-253.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.364 | - |
NAD+ | mitochondrial enzyme | Rattus norvegicus |  |
| 0.443 | - |
NADP+ | enzyme from rough ER | Rattus norvegicus | |
| 0.637 | - |
NADP+ | mitochondrial enzyme | Rattus norvegicus | |
| 0.924 | - |
NAD+ | enzyme from rough ER | Rattus norvegicus | |
| 4.61 | - |
glutamate | mitochondrial enzyme, cofactor NAD+ | Rattus norvegicus | |
| 5.93 | - |
glutamate | enzyme from rough ER, cofactor NAD+ | Rattus norvegicus | |
| 20.7 | - |
glutamate | mitochondrial enzyme, cofactor NADP+ | Rattus norvegicus | |
| 23.8 | - |
glutamate | enzyme from rough ER, cofactor NADP+ | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| rough endoplasmic reticulum | peripheral membrane protein | Rattus norvegicus | 5791 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, enzymes from mitochondria and endoplasmic reticulum | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose, ATP-agarose, Resource Q | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.7 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NADH + NH3 | - |
Rattus norvegicus | L-glutamate + NAD+ + H2O | - |
? | |
| L-glutamate + H2O + NAD(P)+ | - |
Rattus norvegicus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, enzymes from mitochondria and endoplasmic reticulum | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
mitochondrial enzyme loses 80% activity after 20 min, enzyme from endoplasmic reticulum loses 20% activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Rattus norvegicus |
| 9 | 9.5 | glutamte deamination | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | half activity of glutamate deamination compared to pH 9.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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