Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition or activation in the presence of ADP, GTP and leucine | Saccharolobus solfataricus | |
o-phthalaldehyde | 0.1 mM, 98% inhibition after 5 min at 60°C, competitive vs. 2-oxoglutarate and NADH | Saccharolobus solfataricus | |
Phenylglyoxal | 4 mM, 75% inhibition, uncompetitive vs. 2-oxoglutarate, noncompetitive vs. NADH | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, 4°C, 25°C, 6 months, 5% loss of activity | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Saccharolobus solfataricus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
o-phthalaldehyde | vs. 2-oxoglutarate | Saccharolobus solfataricus | |
0.1 | - |
o-phthalaldehyde | vs. NADH | Saccharolobus solfataricus | |
5 | - |
Phenylglyoxal | vs. 2-oxoglutarate | Saccharolobus solfataricus | |
6 | - |
Phenylglyoxal | vs. NADH | Saccharolobus solfataricus |