Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Bos taurus | |
ADP | - |
Urocitellus richardsonii | |
AMP | - |
Bos taurus | |
AMP | - |
Urocitellus richardsonii | |
KCl | activates at 5°C and 37°C | Urocitellus richardsonii | |
leucine | activates at 5°C only | Urocitellus richardsonii | |
NaCl | activates at 37°C only | Urocitellus richardsonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | strong inhibition at 37°C, no inhibition at 5°C | Bos taurus | |
ATP | strong inhibition at 37°C, no inhibition at 5°C | Urocitellus richardsonii | |
GTP | - |
Bos taurus | |
GTP | strong inhibition at 37°C, weak inhibition at 5°C | Urocitellus richardsonii | |
additional information | complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C | Bos taurus | |
additional information | complex inhibition pattern for ATP, GTP, NaCl, KCl, sodium acetate, NaI, and potassium nitrate of forward and reverse reaction at 5°C and 37°C | Urocitellus richardsonii | |
NaCl | - |
Bos taurus | |
Sodium acetate | at 5°C only | Urocitellus richardsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-oxoglutarate | euthermic animal, assay at 37°C | Urocitellus richardsonii | |
0.25 | - |
glutamate | hibernating animal, assay at 5°C | Urocitellus richardsonii | |
0.43 | - |
2-oxoglutarate | hibernating animal, assay at 5°C | Urocitellus richardsonii | |
0.47 | - |
glutamate | assay at 5°C | Urocitellus richardsonii | |
0.5 | - |
glutamate | euthermic animal, assay at 5°C | Urocitellus richardsonii | |
0.98 | - |
2-oxoglutarate | hibernating animal, assay at 37°C | Urocitellus richardsonii | |
1.4 | - |
2-oxoglutarate | - |
Bos taurus | |
1.4 | - |
2-oxoglutarate | assay at 37°C | Urocitellus richardsonii | |
2.03 | - |
glutamate | euthermic animal, assay at 37°C | Urocitellus richardsonii | |
2.6 | - |
glutamate | assay at 37°C | Urocitellus richardsonii | |
3.66 | - |
2-oxoglutarate | euthermic animal, assay at 5°C | Urocitellus richardsonii | |
4.1 | - |
NH4+ | - |
Bos taurus | |
4.1 | - |
NH4+ | assay at 5°C | Urocitellus richardsonii | |
5.2 | - |
glutamate | hibernating animal, assay at 37°C | Urocitellus richardsonii | |
7 | - |
NH4+ | euthermic animal, assay at 37°C | Urocitellus richardsonii | |
10.5 | - |
2-oxoglutarate | - |
Bos taurus | |
10.5 | - |
2-oxoglutarate | assay at 5°C | Urocitellus richardsonii | |
12.1 | - |
NH4+ | hibernating animal, assay at 5°C | Urocitellus richardsonii | |
15.8 | - |
NH4+ | hibernating animal, assay at 37°C | Urocitellus richardsonii | |
17.8 | - |
NH4+ | - |
Bos taurus | |
17.8 | - |
NH4+ | assay at 37°C | Urocitellus richardsonii | |
24.7 | - |
NH4+ | euthermic animal, assay at 5°C | Urocitellus richardsonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59500 | - |
6 * 59500, enzyme from both euthermic and hibernating animals | Urocitellus richardsonii |
320000 | - |
enzyme from hibernating animals, gel filtration | Urocitellus richardsonii |
331000 | - |
gel filtration | Bos taurus |
335000 | - |
enzyme from euthermic animals, gel filtration | Urocitellus richardsonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Urocitellus richardsonii | - |
Richardson's ground squirrel | - |
Purification (Comment) | Organism |
---|---|
enzyme from euthermic and hibernating animals | Urocitellus richardsonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
liver | from euthermic, 37°C body temperature, and hibernating, 5°C body temperature, animals | Urocitellus richardsonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
enzyme from euthermic animals | Urocitellus richardsonii |
201 | - |
enzyme from hibernating animals | Urocitellus richardsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Bos taurus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Urocitellus richardsonii | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 59500, enzyme from both euthermic and hibernating animals | Urocitellus richardsonii |