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Literature summary for 1.4.1.4 extracted from

  • Bhuiya, M.W.; Sakuraba, H.; Ohshima, T.
    Temperature dependence of kinetic parameters for hyperthermophilic glutamate dehydrogenase from Aeropyrum pernix K1 (2002), Biosci. Biotechnol. Biochem., 66, 873-876.
    View publication on PubMed
Search for more literature for 1.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 NADP+ oxidative deamination Aeropyrum pernix K1
0.075
-
 NADPH reductive amination at 70°C Aeropyrum pernix K1
2
-
 2-oxoglutarate reductive amination at 0.6 M NH4Cl Aeropyrum pernix K1
9
-
 2-oxoglutarate reductive amination at 1 M NH4Cl Aeropyrum pernix K1
119
-
 NH3 reductive amination at 70°C Aeropyrum pernix K1
225
-
 L-glutamate oxidative deamination Aeropyrum pernix K1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Aeropyrum pernix K1
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Aeropyrum pernix K1 JCM 9820
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix K1
-
-
-
Aeropyrum pernix K1 JCM 9820
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Aeropyrum pernix K1 L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Aeropyrum pernix K1 JCM 9820 L-glutamate + H2O + NAD+
-
 r
L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 2-oxoglutarate + NADPH + NH3 biphasic kinetic behavior for ammonia r
L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 JCM 9820 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 JCM 9820 2-oxoglutarate + NADPH + NH3 biphasic kinetic behavior for ammonia r

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 no temperature dependence Aeropyrum pernix K1

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Aeropyrum pernix K1
NADPH
-
 Aeropyrum pernix K1