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Literature summary for 1.4.1.4 extracted from

  • Di Fraia, R.; Wilquet, V.; Ciardiello, M.A.; Carratore, V.; Antignani, A.; Camardella, L.; Glansdorff, N.; Di Prisco, G.
    NADP+-dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization, protein and DNA sequence, and relationship to other glutamate dehydrogenases (2000), Eur. J. Biochem., 267, 121-131.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 NADPH reductive amination Escherichia coli
0.043
-
 NADP+ oxidative deamination Psychrobacter sp.
0.045
-
 NADP+ oxidative deamination Escherichia coli
0.2
-
 2-oxoglutarate reductive amination Escherichia coli
2.3
-
 L-glutamate oxidative deamination Escherichia coli
2.5
-
 NH3 reductive amination Escherichia coli
4
-
 NH3 reductive amination Psychrobacter sp.
67.4
-
 L-glutamate oxidative deamination Psychrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 alpha6, 6 * 47000, SDS-PAGE Psychrobacter sp.
49285
-
 alpha6, 6 * 49285, amino acid analysis Psychrobacter sp.
290000
-
 gel filtration Psychrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Escherichia coli
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Psychrobacter sp.
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Escherichia coli DH5-alpha
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Psychrobacter sp. TAD1
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain DH5alpha
-
Escherichia coli DH5-alpha
-
 strain DH5alpha
-
Psychrobacter sp.
-
-
-
Psychrobacter sp. TAD1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Psychrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
78
-
 reductive amination at 20°C Psychrobacter sp.
204.1
-
 oxidative deamination at 20°C Psychrobacter sp.

Storage Stability

Storage Stability Organism
-20°C, 40% v/v glycerol, no activity loss Psychrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Escherichia coli L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Psychrobacter sp. L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Escherichia coli DH5-alpha L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Psychrobacter sp. TAD1 L-glutamate + H2O + NAD+
-
 r
L-glutamate + NADP+ + H2O
-
 Escherichia coli 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Psychrobacter sp. 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Escherichia coli DH5-alpha 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Psychrobacter sp. TAD1 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 47000, SDS-PAGE Psychrobacter sp.
homohexamer alpha6, 6 * 49285, amino acid analysis Psychrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Psychrobacter sp.
55
-
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 irreversible heat inactivation Psychrobacter sp.
61
-
 50% activity after 5 min Escherichia coli
66
-
 50% activity after 5 min Psychrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli
8
-
 oxidative deamination at 20°C Psychrobacter sp.
9
-
-
 Escherichia coli
9
-
 reductive amination at 20°C Psychrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADP+
-
 Psychrobacter sp.
NADPH
-
 Escherichia coli
NADPH
-
 Psychrobacter sp.