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Literature summary for 1.4.1.4 extracted from
- NADP-glutamate dehydrogenase from the halophilic archaeon Haloferax mediterranei: enzyme purification, N-terminal sequence and stability studies (1996), FEMS Microbiol. Lett., 141, 59-63.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal activity at 1-1.5 M | Haloferax mediterranei |  |
| NaCl | optimal activity at 1-1.5 M | Haloferax mediterranei | |
General Stability
| General Stability | Organism |
|---|---|
| in the presence of 4 M NaCl the purified enzyme remains fully active at room temperature for months. The stability is rapidly lost upon lowering the salt concentration | Haloferax mediterranei |
| NaCl and KCl markedly increase the thermostability | Haloferax mediterranei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
NADPH | reductive amination at 25°C | Haloferax mediterranei | |
| 0.018 | - |
2-oxoglutarate | pH 8.5, 40°C | Haloferax mediterranei | |
| 0.34 | - |
2-oxoglutarate | reductive amination at 25°C | Haloferax mediterranei | |
| 0.34 | - |
NH3 | pH 8.5, 40°C | Haloferax mediterranei | |
| 4.2 | - |
NH4+ | reductive amination at 25°C | Haloferax mediterranei | |
| 4.2 | - |
NADPH | pH 8.5, 40°C | Haloferax mediterranei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl | Haloferax mediterranei | |
| NaCl | stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl | Haloferax mediterranei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
alpha6, 6 * 48000, CTAB-PAGE | Haloferax mediterranei |
| 55000 | - |
alpha6, 6 * 55000, SDS-PAGE | Haloferax mediterranei |
| 55000 | - |
6 * 55000, SDS-PAGE | Haloferax mediterranei |
| 320000 | - |
gel filtration | Haloferax mediterranei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Haloferax mediterranei | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Haloferax mediterranei DSM 1411 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | stabilized by glycerol in absence of salt | Haloferax mediterranei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax mediterranei | - |
- |
- |
| Haloferax mediterranei DSM 1411 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax mediterranei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
reductive amination at 40°C | Haloferax mediterranei |
| 60 | - |
pH 8.5, 40°C | Haloferax mediterranei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, 20% v/v glycerol, 476 h, 50% activity | Haloferax mediterranei |
| fully active at 4 M concentration NaCl at room temperature for months | Haloferax mediterranei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH3 + NADPH + H+ | - |
Haloferax mediterranei | L-glutamate + H2O + NADP+ | - |
? | |
| 2-oxoglutarate + NH3 + NADPH + H+ | - |
Haloferax mediterranei DSM 1411 | L-glutamate + H2O + NADP+ | - |
? | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Haloferax mediterranei | L-glutamate + NADP+ | - |
? | |
| 2-oxoglutarate + NH4+ + NADPH | - |
Haloferax mediterranei DSM 1411 | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Haloferax mediterranei | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Haloferax mediterranei DSM 1411 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 55000, SDS-PAGE | Haloferax mediterranei |
| homohexamer | alpha6, 6 * 55000, SDS-PAGE | Haloferax mediterranei |
| homohexamer | alpha6, 6 * 48000, CTAB-PAGE | Haloferax mediterranei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP(H)-specific glutamate dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Haloferax mediterranei |
| 60 | - |
- |
Haloferax mediterranei |
| 60 | - |
reductive amination | Haloferax mediterranei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the thermostability decreases significantly as the salt concentration is lowered | Haloferax mediterranei |
| 67 | - |
t1/2 in presence of 2 M KCl is about 27 min, while in 4 M KCl the t1/2 is 70 h | Haloferax mediterranei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Haloferax mediterranei |
| 8.5 | - |
reductive amination | Haloferax mediterranei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Haloferax mediterranei | |
| NADPH | - |
Haloferax mediterranei | |
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